Applicationof warangalone to preparation of cervical cancer preventing and/or treating drug
A technology of climbing deer isoflavone and cervical cancer, which is applied in the field of application of climbing deer isoflavone in the preparation of drugs for preventing and/or treating cervical cancer, and can solve the problems of unclear molecular mechanism and unclear effect of inhibiting tumor cells , to achieve the effect of safe and reliable ingredients and great application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Example 1 Warangalone anti-tumor activity in vitro
[0027] The half-inhibitory concentration (IC) of cells 50 ) is one of the commonly used methods for evaluating the ability of drugs to inhibit tumor proliferation. The present invention selects 8 kinds of human normal cells and tumor cells derived from different tissues to detect the anti-tumor ability of Warangalone, and uses the MTT method to measure the effect of Warangalone on cell proliferation. The drug action time is 72h, and the results are shown in Table 1: HeLa Human cervical cancer cells, HepG2 human liver cancer cells, M321 and MCF-7 human breast cancer cells, LO2 normal human liver cells, and chem-5 normal brain glial cells. The growth of tumor cells treated with Warangalone was inhibited to varying degrees. Through analysis, it can be seen that the IC of Warangalone on several different tumor cells of cervical cancer, breast cancer and liver cancer 50 The range is between 13.3-29.6 μ M, which is signif...
Embodiment 2
[0030] Example 2 Time Effect of Warangalone on HeLa Cell Proliferation Inhibition
[0031] MTT method was used to evaluate the growth of HeLa cells with different concentrations of Warangalone (0, 5, 10, 15, 20, 25, 30 μM) for 12h, 24h, 48h and 72h. The result is as figure 1 As shown, the growth inhibitory effect of Warangalone on HeLa cells was dose-dependent, and the IC of the inhibitory effect on HeLa cell proliferation at 12h, 24h, 48h and 72h 50 The values were 37.82±0.42μM, 20.97±0.55μM, 17.38±0.44μM, 13.31±0.26μM, and Warangalone's effect on HeLa cells for 48 hours was more obvious in apoptosis.
Embodiment 3
[0032] Embodiment 3 Warangalone changes HeLa cell morphology
[0033] HeLa in the logarithmic phase was spread in a 6-well plate. After the cells adhered to the wall, different concentrations of drugs were added to act for 24 hours and 48 hours respectively. The morphology of the cells was observed and photographed under an inverted microscope. Such as figure 2 As shown, compared with the cells of the blank control group, with the increase of the drug concentration of Warangalone, the HeLa cells not only decreased in number, but also changed their shape significantly with the increase of the concentration. The shape change of the low concentration of 10μM cells is not obvious, but the number of cells is reduced; at 20μM, the shape of most cells changes, from diamond to spindle and round, and the ability to adhere to the wall decreases; at 30μM, most of the cells are round , the light transmittance increases and gradually falls off from the wall. The results showed that Wara...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More