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A microfluidic SERS chip for PDMS self-absorption sampling and preparation method thereof

A microfluidic and chip technology, which is applied in chemical instruments and methods, laboratory containers, material analysis through electromagnetic means, etc., can solve the problem of poor adhesion between SERS substrate and microchannel, easy leakage of samples, space problems, etc. Narrow and other problems, to achieve the effect of shortening the detection time, preventing mutual cross-sampling, and preventing cross-sampling

Active Publication Date: 2019-04-30
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned defects, an object of the present invention is to provide a microfluidic SERS chip for PDMS self-absorption sampling, which can not only solve the technical problem of leakage caused by poor bonding between the SERS substrate and the microchannel, but also allow PDMS self-absorption sampling The method can overcome the technical defects of small space, inconvenient operation, and easy leakage of samples in microstructure injection, negative pressure pumping and other methods of sample injection. In addition, it is simple and convenient to make, low in cost, small in size, and easy to carry

Method used

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  • A microfluidic SERS chip for PDMS self-absorption sampling and preparation method thereof
  • A microfluidic SERS chip for PDMS self-absorption sampling and preparation method thereof
  • A microfluidic SERS chip for PDMS self-absorption sampling and preparation method thereof

Examples

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Effect test

Embodiment 1

[0047] Example 1 Preparation of PDMS self-aspiration microfluidic SERS chip

[0048] Step 1: prepare the ITO substrate: use the three-electrode system of the VersaSTAT3 electrochemical workstation, use the platinum electrode as the counter electrode, the saturated calomel electrode as the auxiliary electrode, select a clean 10mm×10mm ITO conductive glass as the working electrode, and use 0.3mol / L KNO 3 10mL of a mixed solution of silver ammonia and 1.6mmol / L is the electrolyte, and at room temperature (25°C ± 5), using electrodeposition technology, depositing at -1.0V for 10s to nucleate nano-Ag on the surface, and then Depositing at -0.4V potential for 2000s, a uniformly distributed nano-Ag@ITO substrate was obtained; by HAuCl 4 Optimization of solution concentration and soaking time to obtain HAuCl at 0.1mmol / L 4 Under the optimal conditions of soaking for 2 minutes, the Nano-Ag&Au@ITO reinforced substrate was prepared. See the structure figure 2 ;

[0049] Step 2 Attac...

Embodiment 2

[0053] Example 2 Detection of human serum samples

[0054] The PDMS self-absorption microfluidic SERS chip prepared in Example 1 was used for SERS test analysis on human serum samples. Put the microfluidic SERS chip in Example 1 into the vacuum box to evacuate for 20 minutes, then use a 10 μL pipette to draw 6 different human serum samples, drop them into 6 different injection holes, and wait for 10 minutes. Replenish the injection port liquid at all times, and when the microchannel is filled with the sample solution, remove the PDMS elastomer compact (the adhesion between the PDMS elastomer and the PDMS cover slip is greater than the adhesion between the PDMS cover slip and the double-sided adhesive tape). The force should be small), and the ocean Raman spectrometer is used for testing. The experimental conditions are that the laser wavelength is 785nm, the laser power is 3.5mw, the integration time is 3s, and the number of integrations is 1 time. Ionized water for SERS spec...

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Abstract

The invention belongs to the technical field of surface-enhanced Raman spectral analysis and micro-fluidic chip analysis, and in particular relates to a micro-fluidic SERS chip with PDMS self-priming sampling and a preparation method thereof. The method can solve the technical problem of liquid leakage resulted from unstable fit between an SERS substrate and a microchannel, and the PDMS self-priming sampling mode can overcome the technical defects of narrow space, inconvenient operation, liquid leakage of a sample and the like existing in sampling modes of injection, negative pressure pumping and the like in a microstructure. In addition, the micro-fluidic SERS chip with PDMS self-priming sampling is simple and convenient in production, low in cost, smaller in dimension and convenient for carrying.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman spectrum analysis and microfluidic chip analysis, and in particular relates to a microfluidic SERS chip for PDMS self-absorption sampling and a preparation method thereof. Background technique [0002] Microfluidic chip analysis technology has attracted much attention in the analysis and detection of biochemical samples due to its advantages of controllable liquid flow, minimal consumption of samples and reagents, high analysis efficiency, low cost, and high throughput. At present, the analysis methods mainly include electrochemical detection, fluorescence detection and other methods, but these methods have problems such as complicated sample pretreatment, adding indicators, and less information. As a non-destructive detection method, Raman spectroscopy has special advantages in the detection of biochemical solutions because it will not cause irreversible damage to the sample during...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/65G01N27/416
CPCB01L3/502707B01L2200/12B01L2300/0887B01L2300/16G01N21/658G01N27/416
Inventor 徐溢郑祥权陈李李刚
Owner CHONGQING UNIV
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