A fluorescent probe for detecting intracellular peroxynitrite ion and its synthesis method

Abstract

The invention provides a fluorescent probe for detecting peroxynitrite in a cell. The structural formula of the fluorescent probe is shown in the description, wherein R1 and R2 are selected from hydrogen, linear chain alkyl containing 1 to 5 carbon atoms or naphthenic base containing 3 to 8 carbon atoms respectively and separately; R3 is selected from a cyano group or an ester group. The fluorescent probe has high peroxynitrite selectivity, high cell permeability, extremely-short response time, extremely high detection sensitivity and no toxic and side effects to the cell per se, and is suitable for detecting the concentration change of the peroxynitrite in the cell. The fluorescent probe synthetic method is simple, is high in yield, and is suitable for large-scale production.

Description

technical field

[0001] The invention relates to the field of chemistry, in particular to a fluorescent probe for detecting intracellular peroxynitrite ions and a synthesis method thereof. Background technique

[0002] Since the discovery in the 1980s that NO can be synthesized by enzymes in vivo, people began to study the biomedical aspects of NO. The Nobel Prize in 1998 was awarded to scientists who discovered the special function of NO. It is particularly worth mentioning that NO not only acts as a messenger molecule in biological tissues, NO and O 2 - It can also form peroxynitrite (ONOO - ). Peroxynitrite ion is more effective than NO and O 2 - A potent cytotoxic substance with stronger oxidation and wider effects, which can diffuse a long distance from the production site to reach the target site, causing extensive damage, and is a powerful oxidant that causes cell and tissue damage. Peroxynitrite ion can act on macromolecular substances such as enzymes, proteins...

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