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Culture medium, culture medium-containing kit and application thereof

A culture medium, a kit technology, applied to the culture medium, the kit containing the same, and the application fields thereof, can solve the problems of high personnel cost, long detection time, and high cost of consumables

Active Publication Date: 2017-10-24
CREATIVE MICROBIOLOGICALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, it can be seen that the existing culture medium still has the problems of high personnel cost, high cost of consumables, and long detection time in the laboratory

Method used

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  • Culture medium, culture medium-containing kit and application thereof
  • Culture medium, culture medium-containing kit and application thereof
  • Culture medium, culture medium-containing kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of culture medium

[0028] The following provides an exemplary embodiment of the formulation of the culture medium according to the present disclosure, and illustrates the content of each component included in each liter, wherein, Example 1A is a culture solution for group B streptococci producing carrot color, which is hereinafter referred to as Embodiment 1A liquid culture medium or embodiment 1A medium; Embodiment 1B is that group B streptococcus produces carrot color plate culture medium, hereinafter referred to as embodiment 1B plate culture medium or embodiment 1B culture medium; Embodiment 1C It is the culture medium for the sample transport / enrichment / identification kit used in the group B Streptococcus transport culture device, which is referred to as the medium of Example 1C hereinafter.

[0029]

[0030] When preparing the liquid culture medium of Example 1A, the above-mentioned ingredients were divided into two parts for prep...

preparation Embodiment 1

[0035] When preparing the culture medium for the sample delivery / enrichment / identification kit in Example 1C, the steps are similar to those for preparing the liquid culture medium in Example 1A, but add 4 to 6 g of agar powder to the first part, and when subpackaging, it is Dispense 4.8mL of still liquid culture medium into sterile sampling tubes and sterilize with gamma rays.

[0036] Figure 1a , b, and c respectively show the color development of the medium in Example 1A, Example 1B, and Example 1C inoculated with β-hemolytic GBS and other bacteria. As shown in the figure, β-hemolytic GBS can produce light orange to red pigment, while other strains do not show light orange to red pigment.

Embodiment 2

[0037] Embodiment 2: Evaluate the effectiveness of embodiment 1B plate culture medium to differentiate GBS

[0038] In order to explore the clinical applicability of the plate medium disclosed in this study, 160 strains of test bacteria (comprising 120 strains of clinical isolates of group B streptococci, 8 strains of non-group B streptococci and 32 strains of other non-streptococcus Species) for efficacy evaluation, the items include specificity, sensitivity, positive predictive value, negative predictive value and efficiency (efficiency) presented in different culture environments, inoculation methods and culture time. In order to understand the similarities and differences between the performance of the disclosed plate medium and the commercially available CHROMagar StrepB (CHROMagar Company, France), in addition to evaluating the sensitivity and specificity of CHROMagar StrepB with the same test bacteria, we further simulated the mixed bacteria of clinical samples. These t...

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Abstract

The invention provides a culture medium for detecting group B streptococcus (GBS). The culture medium comprises an enrichment formulation, polysaccharides and a microbial screening agent. The invention further provides a kit for detecting the group B streptococcus. The kit comprises the culture medium and one or more containers for accommodating the culture medium; and the one or more containers are independently selected from a group consisting of a sampling tube, a test tube and a culture dish. The culture medium disclosed by the invention has the advantages of low cost, simplicity and convenience in storage, relatively short detection time, sensitivity and accuracy during distinguishing of the GBS.

Description

technical field [0001] The present disclosure pertains to media for culturing microorganisms. More specifically, the present disclosure relates to a culture medium for detecting Group B Streptococcus (Group B Streptococcus, GBS for short, ie Streptococcus agalactiae, also known as Group B Streptococcus). The present disclosure also provides the use of the culture medium. Background technique [0002] Group B Streptococcus (Group B Streptococcus, referred to as GBS, that is, Streptococcus agalactiae (Streptococcus agalactiae), also known as Streptococcus B), is a Gram-positive coccus that often inhabits the human intestinal tract and genitourinary tract. Research by the National Health Administration of the Ministry of Health and Welfare of the Executive Yuan shows that about 18 to 20% of pregnant women in Taiwan have GBS colonization in the reproductive tract. However, pregnant women with bacteria usually do not have clinical symptoms, but because newborns have low resistan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12R1/46
CPCC12Q1/045C12Q1/14
Inventor 蔡文城
Owner CREATIVE MICROBIOLOGICALS