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Drug for inhibiting intra-cellular replication in porcine reproductive and respiratory syndrome virus (PRRSV)

A technology for respiratory syndrome and pig reproduction, applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problem of not designing effective antiviral drugs, and achieve good market value and clinical application prospects Effect

Inactive Publication Date: 2017-11-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the in-depth study of PRRSV molecular biology, there is no report on the design of effective antiviral drugs for different stages of the PRRSV replication cycle.

Method used

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  • Drug for inhibiting intra-cellular replication in porcine reproductive and respiratory syndrome virus (PRRSV)
  • Drug for inhibiting intra-cellular replication in porcine reproductive and respiratory syndrome virus (PRRSV)
  • Drug for inhibiting intra-cellular replication in porcine reproductive and respiratory syndrome virus (PRRSV)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 125

[0031] Example 1 Inhibition experiment of 25HC to PRRSV

[0032] 1. Method

[0033] 1.1 Indirect immunofluorescence test

[0034] MARC-145 cells infected with PRRSV were fixed with pre-cooled absolute ethanol at room temperature for 30 minutes (30minutes, 30min), the mouse PRRSV N protein monoclonal antibody (1:1000 dilution) was used as the primary antibody, and the effect was 2 hours at 37°C ( 2hours, 2h), then washed 3 times with PBS, FITC-conjugated goat anti-mouse IgG (H+L) (1:200 dilution) was used as the secondary antibody, acted at 37°C for 1h, washed 3 times with PBS, and finally used a fluorescent microscope (Nikon , Japan) observed.

[0035] 1.2 Determination of virus titer

[0036] Spread MARC-145 cells in a 96-well plate, and infect PRRSV 48 hours later, collect the cells 48 hours post-infection (48hpi), and measure the virus titer by using microtiter method combined with indirect immunofluorescence test, and the results are expressed as TCID 50 / ml (50% tissu...

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Abstract

The invention provides a drug for inhibiting intra-cellular replication in porcine reproductive and respiratory syndrome virus (PRRSV). Through cellular-level research on the effect of 25-hydroxycholesterol (25HC) on the PRRSV, the result shows that the 25HC can obviously inhibit replication of multiple strains of the PRRSV, adsorption and entry phases of replication cycles of the PRRSV can be inhibited without affecting synthesis of viral genomes and release of virus particles, and the 25HC does not have a direct inactivation function on the virus particles of the PRRSV. The 25HV is hopefully developed into a candidate drug for preventing the PRRSV. The new drug is provided for preventing and treating the PRRSV, and good market value and clinical prospect are achieved.

Description

technical field [0001] The invention relates to the field of antiviral drugs, in particular to a drug for inhibiting intracellular replication of porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV), which seriously endangers the global pig industry. The disease is characterized by reproductive failure in sows and respiratory disease in pigs of all ages. PRRSV is a single-strand positive-sense RNA virus with an envelope, belonging to the Arteriviridae Arterivirus genus of the order Neveviridae. According to different genetic characteristics and antigenicity, it is divided into genotype I and genotype II. The virus has a high degree of variation Its continuous evolution and mutation in the field lead to the production of many recombinant and mutate...

Claims

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Application Information

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IPC IPC(8): A61K31/575A61P31/14
CPCA61K31/575
Inventor 杨汉春董泓盖新娜周磊郭鑫韩军
Owner CHINA AGRI UNIV
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