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Method for determining dtpa-ca in rat plasma biological samples

A biological sample, plasma technology, applied in the field of biomedicine, can solve the problem of insufficient lead to evaluate the in vivo behavior of DTPA

Active Publication Date: 2019-09-24
万舒(北京)医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In such a wide range of application environments, it is obviously not enough to evaluate the in vivo behavior of DTPA, a metal chelator, only by examining the distribution and content of lead in the body

Method used

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  • Method for determining dtpa-ca in rat plasma biological samples
  • Method for determining dtpa-ca in rat plasma biological samples
  • Method for determining dtpa-ca in rat plasma biological samples

Examples

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Embodiment Construction

[0062] The following examples of the present invention are provided for illustrative purposes only and are not intended to, nor should they be construed as, limiting the present invention in any way. Those skilled in the art will recognize that routine changes and modifications can be made to the following examples without departing from the spirit or scope of the invention.

[0063] One purpose of the present invention is to better understand the in vivo disposal rules of DTPA-Ca. The present invention has established and verified the liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for quantitatively detecting the content of DTPA-Ca in biological samples. , while investigating the pharmacokinetics and bioavailability of DTPA-Ca in rats.

[0064] 1 Summary

[0065] Ca-DTPA powder spray (pentetate calcium trisodium salt, DTPA-CaNa 3 ) is intended to be used clinically as a radionuclide expulsion drug for the removal of radionuclides in the lungs. In order ...

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Abstract

The invention relates to a method for measuring DTPA-Ca in a rat plasma biological sample. Specifically, according to the method for measuring DTPA-Ca in the rat plasma biological sample in the invention, the content of the DTPA-Ca in the rat plasma biological sample through treatment is measured by using liquid chromatogram-tandem mass spectrometry, and the method comprises the following steps: (1) treatment of the biological sample; (2) liquid chromatogram-tandem mass spectrometry measurement; and (3) data processing and analysis. The method in the invention has excellent effects in the specification of the invention, for example, the method disclosed by the invention has an excellent chromatograph effect and a high target object recycle rate.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a reliable method for measuring the content of the above-mentioned metal chelating agent in biological samples such as blood, urine, etc. Therapeutic effects of these metal chelators or their behavior in vivo. [0002] In particular, the present invention relates to a method for the determination of DTPA-Ca in a biological sample of rat plasma. Background technique [0003] The harm of lead pollution and lead poisoning to human health has been widely concerned. Lead is a non-essential element for the human body. It enters the human body for absorption mainly through the respiratory tract and digestive tract. It can accumulate in the body and has toxic effects on various systems and organs throughout the body. It mainly affects the nervous, digestive, and blood systems. Lead pollution and lead poisoning need to be addressed. [HaoFT, Du XQ, Niu YM, et al. Progress in research o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N33/50
Inventor 郭继芬孟繁华虞林李照丰
Owner 万舒(北京)医药科技有限公司