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A kind of preparation method of magnetic cellulose microsphere immobilized lipase catalyst

A technology for immobilizing lipase and cellulose microspheres, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., can solve the problem of limiting the large-scale application of lipase, difficulty in recovery and separation and purification , lipase inactivation and other issues, to achieve the effect of recycling and reuse, low price and high selectivity

Active Publication Date: 2021-06-01
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, lipase is easily inactivated by the environment, has poor stability, and is difficult to recover and separate and purify. These shortcomings limit the large-scale application of lipase in industrial production.

Method used

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  • A kind of preparation method of magnetic cellulose microsphere immobilized lipase catalyst
  • A kind of preparation method of magnetic cellulose microsphere immobilized lipase catalyst

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of regenerated cellulose microspheres by hot melt adhesive conversion method: Soak absorbent cotton with a certain amount of lye for 2-5 hours, then drain the solution, leave it for aging for 40-50 hours, add appropriate amount of CS 2 Sulfonate at 25-40°C for 6-10 hours, add appropriate amount of lye and CaCO 3 Stir evenly, pour into 400~600mL transformer oil containing 0.05~0.1g potassium oleate, 0.2~0.4g Span 60, stir at room temperature for 0.5~2h, then slowly raise the temperature to 70~90℃, and cure for 1.5~3.5h, Pour out the oil, wash the solid with a large amount of hot water until there is no oil, and obtain cellulose microspheres; add the cellulose microspheres to an appropriate amount of dilute hydrochloric acid solution, shake until there are no bubbles, pour out the liquid, add new dilute hydrochloric acid, and repeat several times , until the liquid becomes acidic, and the porous cellulose microspheres are prepared.

Embodiment 2

[0021] Chemical modification: add porous cellulose microspheres to epichlorohydrin, add an appropriate amount of lye to react, and prepare epoxidized cellulose microspheres; add appropriate amount of epoxidized cellulose balls to 8-12mL In the amine modification reagent, react at 40-60° C. for 8-12 hours to obtain amino-modified cellulose microspheres.

Embodiment 3

[0023] Preparation of magnetic cellulose microspheres by co-precipitation: add amino-modified cellulose microspheres to 100-200mL Fe 3+ and Fe 2+ The mixed liquid is vacuum filtered at 0-25°C for 1 hour, ammonia water is added to adjust the pH of the liquid to 9-11, and coprecipitation is carried out at 50-60°C for 1-3 hours to prepare the magnetic cellulose microsphere matrix.

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Abstract

The present invention relates to a preparation method of magnetic cellulose microsphere immobilized lipase catalyst, and the purpose is to provide a simple, economical and efficient method for preparing immobilized lipase catalyst. The technology and method adopted are as follows: As raw material, solid calcium carbonate particles were used as porogen, hot melt adhesive conversion method, combined with reverse suspension technology, was used to prepare porous regenerated cellulose microspheres, and then epoxidized, aminated and grafted to obtain microporous Magnetic cellulose microspheres were prepared for co-precipitation reaction in the reactor, and lipase molecules were immobilized on the surface and micropores of the magnetic cellulose microspheres by covalent binding method. This method has rich and easy-to-obtain raw materials, and the prepared magnetic cellulose carrier has small size, uniform distribution, and large specific surface area. Used in medicine, environment, energy and other fields.

Description

technical field [0001] The invention relates to an immobilized lipase catalyst with magnetic cellulose microspheres as a carrier and a preparation method thereof, belonging to the technical field of immobilized enzyme biocatalyst preparation technology. Background technique [0002] Lipase, also known as triacylglycerol hydrolase, is an enzyme that catalyzes the hydrolysis of esters to produce fatty acids and glycerol. Lipase is a typical reversible enzyme, which can not only catalyze the hydrolysis of oil in the aqueous phase, but also catalyze the ester synthesis reaction in the non-aqueous phase, and these two reactions can be combined in a continuous manner to trigger a A series of reactions, such as exchange reaction, acidolysis reaction, alcoholysis reaction and ammonolysis reaction, etc. It can efficiently catalyze the reaction under mild conditions of normal temperature and pressure, and has specificity, and has more and more applications in medicine, environment, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/12
CPCC12N9/20C12N11/12C12Y301/01003
Inventor 付玉杰韩盈枝王希清焦骄罗猛刘志国王微
Owner NORTHEAST FORESTRY UNIVERSITY