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Production method of podostroma cornu-damae cultivating strain

A production method and technology of sarcoidium, applied in the field of production of sarcoidium cultured strains, can solve the problems of unseparated and purified strains and cultivation, and achieve the effect of simple operation

Inactive Publication Date: 2017-11-17
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Corynebacterium meatus is not yet known and understood by the vast majority of people in China, and even most people who are engaged in fungal research have no concept of this species, and only a few species have been described and recorded in China, and no isolated and purified bacteria have been found so far. Reports on the use of species, cultivation, etc.

Method used

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  • Production method of podostroma cornu-damae cultivating strain

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Embodiment 1

[0018] (1) Production of wheat grain medium: select wheat grains with full grains, no broken grains, and no moths, soak them in pure water for 12 hours, take them out and dry them, add 0.5% quicklime and 0.5% gypsum (the percentage is based on the quality of wheat grains) , mix well with the dried wheat grains and put them into glass bottles. Each bottle is filled with about half a bottle of well-mixed wheat grains, and then add 20 ml of nutrient solution (1% peptone solution, 1% beef extract to dissolve) Liquid, 1% KH 2 PO 4 solution mixture), adjust the pH to 7, and wait for sterilization;

[0019] (2) Sterilization: Sterilize the wheat grain culture medium in the glass bottle, autoclave at 121°C-123°C for 90-120min, take it out, put it into the ultra-clean workbench to cool to normal temperature, and wait for inoculation;

[0020] (3) Inoculation: Get 3-5 pieces (7-8mm in diameter) of the cultivated sarcoidium original species, and disperse inoculate them in the glass bot...

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Abstract

The invention relates to a production method of a podostroma cornu-damae cultivating strain. The method comprises the steps of 1 wheat grain culture medium manufacturing, 2 sterilizing, 3 inoculating and 4 cultivating. The method is easy to implement and can be used for cultivating potential novel resource food podostroma cornu-damae, and a pioneer for researching, developing and utilizing the potential novel resource food podostroma cornu-damae in China is created.

Description

technical field [0001] The invention belongs to the field of edible and medicinal fungi, and in particular relates to a production method of the cultivated strain of Coryne sarcoidium. Background technique [0002] my country is one of the countries with the richest biodiversity. It is estimated that there are 100,000 species of edible and medicinal fungi in China, but only about 1,000 species have been known, and only about 100 species have been used. It can be seen that most wild edible and medicinal fungi Bacteria are not discovered, recognized and utilized. Therefore finding better edible and medicinal fungi is one of the important tasks of Chinese edible and medicinal fungi workers. [0003] Podostroma sp. is a special species with 16 ascospores. There are currently 20 records of Podostroma sp. in the world, which shows how precious Podostroma is. Corynebacterium meatus is not yet known and understood by the vast majority of people in China, and even most people who ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 李传华张劲松奚莉萍陈明杰汪虹
Owner SHANGHAI ACAD OF AGRI SCI