Cotton cytoplasm male sterility 'three-way hybrid' high-efficiency seed production method
A three-cross and cytoplasmic technology, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of low efficiency, high investment, small scale, etc., and achieve high efficiency
Inactive Publication Date: 2017-11-17
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology
[0004] At present, the cotton hybrid seed production method mainly relies on manual removal of stamens, which has low efficiency, small scale, high investment and high risk, and the purity of seeds cannot be guaranteed.
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[0027] The first main technical concept of the present invention is based on the "three-line" seed production method, inserting another maintainer line (referring to the sterile line with the sterile line) one generation before the test crossing between the sterile line and the restorer line. The corresponding maintainer line with strong combining ability has different traits compared to the original one) to carry out a backcross, so that the sterile line produced with the restorer line is a new sterile line in a heterozygous state (containing two maintainer line genes), and finally Hybrid cotton seeds with higher production performance were produced.
[0028] The second main technical idea of the present invention is to provide a new high-efficiency seed production method of mixed planting for the defects of low pollination efficiency and manual intervention of existing sterile lines and restorer lines. It is to use a photon new male sterile line with a pair of relative tra...
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The invention discloses a cotton cytoplasm male sterility 'three-way hybrid' high-efficiency seed production method. The method comprises the following steps: producing a 'three-line' sterile line by utilizing an original maintainer line with high combining ability; backcrossing once by utilizing another high-quality maintainer line with character difference, and producing a novel 'three-line' sterile line containing two maintainer line genes; and performing test cross with a 'three-line' restoring line to prepare 'three-way hybrid', and screening and identifying the 'three-way hybrid' with strong competitive edge through the test. According to the seed production method disclosed by the invention, the cytoplasmic-nucleic male sterility 'three-line three-way hybrid' hybrid cotton at high yield, high efficiency, high purity and low cost can be successfully bred, and compared with sterile line single cross hybrid F1 seeds, the seeds produced by the invention have excellent commodity production performance.
Description
technical field [0001] The invention relates to the field of cotton breeding, in particular to a cotton cytoplasmic sterile "three-cross" high-efficiency seed production formula. Background technique [0002] The premise of heterosis utilization of cotton cytoplasmic male sterility is "three lines" matching, that is, the matching of sterile line (A), maintainer line (B) and restorer line (R). The "three lines" should be fertile, the stamens of the maintainer line and the restorer line are normally fertile, and self-fertilization can reproduce offspring, and the hybrids can also self-fertilize; the sterile line itself cannot produce active pollen grains, and its Propagation is to realize the reproduction of the sterile line through the supply of pollen by the maintainer line; when the sterile line and the restorer line (usually 3:1 or 1:1) are planted at intervals, due to the role of pollinators, the sterile line obtains pollen from the restorer line The resulting seed is th...
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Login to View More IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 周晓箭杨代刚马雄风裴小雨李威刘艳改贺昆仑张飞张文生
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI