A kind of degradable polylactic acid/silk fibroin/chitosan composite nerve guide and its preparation method

Abstract

The invention belongs to the field of medical biological materials, and particularly relates to a degradable polylactic acid / fibroin / chitosan composite nerve conduit and a preparation method thereof. The composite nerve conduit is prepared from polylactic acid, silk fibroin and chitosan, the polylactic acid and the silk fibroin are prepared into a nerve conduit inner layer membrane through the electrostatic spinning method, the polylactic acid is prepared into a nerve conduit outer layer membrane on the inner layer of the conduit through the electrostatic spinning method, the prepared nerve conduit is immersed into a chitosan solution through a solution extraction method, the nerve conduit is placed in a vacuum drying box for solution volatilization, and the polylactic acid / fibroin / chitosan composite nerve conduit is prepared. The composite nerve conduit has good mechanical compressive properties, good biocompatibility, cell affinity and proper degradation performance so that the activity of cells can be effectively improved and formation of nerve scars and nerve tumors can be inhibited; the composite nerve conduit has a bionic structure for simulating an extracellular matrix and is beneficial to adhesion, proliferation and migration of cells in the conduit.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, in particular to a degradable polylactic acid / silk fibroin / chitosan composite nerve guide and a preparation method thereof. Background technique [0002] Peripheral nerve injury is a common disease in daily life. Short-distance nerve defects can be repaired by autologous transplantation, but if the distance of nerve defects is too long, autologous transplantation will face the problem of insufficient donors, donor site infection, scar formation, etc. It also limits the clinical application of autologous transplantation in peripheral nerve repair. Therefore, research on artificial nerve guides is very urgent. The materials used in traditional artificial nerve guides are not easy to degrade, have poor biocompatibility, and the preparation methods are relatively backward, which cannot well meet the needs of actual nerve repair. [0003] In recent years, with the continuous research on ...

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Problems solved by technology

[0002] Peripheral nerve injury is a common disease in daily life. Short-distance nerve defects can be repaired by autologous transplantation, but if the distance of nerve defects is too long, autologous transplantation will face the problem of insufficient donors, donor site infection, scar formation, etc. It also limits the clinical application of autologous transplantation in peripheral nerve repair

Therefore, the research on artificial nerve guides is very urgent. The materials used in traditional artificial nerve guides are not easy to degrade, have poor biocompatibility, and the preparation methods are relatively backward, which cannot well meet the needs of actual nerve repair.

[0003] In recent years, with the continuous research on artificial nerve conduits, great progress has been made, but there are still many problems, such as insufficient pressure resistance of nerve conduits, easy to be squeezed by surrounding tissues, causing conduit collapse; biocompatibility Poor sex, leading to inflammation, easy to form nerve scars, tissue adhesion and other problems

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