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Preparation method for ice nucleus active bacteria compound for preventing and controlling insect attack

A technology of ice-nucleating active bacteria and composition, which is applied in the field of agricultural crop pest control, can solve the problems that cannot be popularized and used, and achieve the effects of pollution-free pest control, avoiding competition, and uniform spraying

Inactive Publication Date: 2017-11-24
黎昊源生物工程(山东)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few related reports in the prior art, and the only scheme for preventing and controlling pests by ice-nucleating active bacteria can only play a role on a single pest, and cannot be popularized and used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method for an ice nucleation active bacterial composition for preventing and controlling insect pests, comprising:

[0031] Step (1), fermenting ice nucleation active bacteria;

[0032] Wherein, the active bacteria of ice nucleus include Pseudomonas syringae, Erwinia pineapple, and Xanthomonas;

[0033] The fermentation method is:

[0034] Step I, preparation of liquid medium: medium components are: glucose 20g / L, peptone 9g / L, beef extract 7g / L, potato flour 10g / L, corn flour 10g / L, MgSO 4 ·7H 2 O 0.1g / L, K 2 HPO 4 0.3g / L, NaCl 0.03g / L;

[0035] Step II, drop part of the liquid culture medium of step I into the culture bottle, shake, and fully mix the Pseudomonas syringae, Erwinia pineapple, and Xanthomonas in the culture bottle with the liquid culture medium;

[0036] Step III, using ultraviolet rays to irradiate the fermentation reactor, then purging the reactor with nitrogen to provide a sterile environment, then adding the materials processed i...

Embodiment 2

[0039] A preparation method for an ice nucleation active bacterial composition for preventing and controlling insect pests, comprising:

[0040] Step (1), fermenting ice nucleation active bacteria;

[0041] Wherein, the active bacteria of ice nucleus include Pseudomonas syringae, Erwinia pineapple, and Xanthomonas;

[0042] The fermentation method is:

[0043] Step I, preparation of liquid medium: medium components are: glucose 12g / L, peptone 5g / L, beef extract 2g / L, potato flour 5g / L, corn flour 1g / L, MgSO 4 ·7H 2 O 0.01g / L, K 2 HPO 4 0.01g / L, NaCl 0.01g / L;

[0044] Step II, drop part of the liquid culture medium of step I into the culture bottle, shake, and fully mix the Pseudomonas syringae, Erwinia pineapple, and Xanthomonas in the culture bottle with the liquid culture medium;

[0045] Step III, using ultraviolet rays to irradiate the fermentation reactor, then purging the reactor with nitrogen to provide a sterile environment, then adding the materials processed i...

Embodiment 3

[0049] A preparation method for an ice nucleation active bacterial composition for preventing and controlling insect pests, comprising:

[0050] Step (1), fermenting ice nucleation active bacteria;

[0051] Wherein, the active bacteria of ice nucleus include Pseudomonas syringae, Erwinia pineapple, and Xanthomonas;

[0052] The fermentation method is:

[0053] Step I, preparation of liquid medium: medium components are: glucose 15g / L, peptone 6g / L, beef extract 6g / L, potato flour 6g / L, corn flour 8g / L, MgSO 4 ·7H 2 O 0.02g / L, K 2 HPO 4 0.03g / L, NaCl 0.02g / L;

[0054] Step II, drop part of the liquid culture medium of step I into the culture bottle, shake, and fully mix the Pseudomonas syringae, Erwinia pineapple, and Xanthomonas in the culture bottle with the liquid culture medium;

[0055] Step III, using ultraviolet rays to irradiate the fermentation reactor, and then purging the reactor with nitrogen to provide a sterile environment, then adding the materials processed...

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Abstract

The invention discloses a preparation method for an ice nucleus active bacteria compound for preventing and controlling insect attack. The method is characterized by comprising the following steps: (1) fermenting ice nucleus active bacteria which include pseudomonas syringae, pineapple erwinia and xanthomonas and (2) uniformly mixing the acquired ice nucleus active bacteria with a compound surfactant, thereby acquiring the ice nucleus active bacteria compound for preventing and controlling insect attack. A large amount of pseudomonas syringae, pineapple erwinia and xanthomonas fermented according to the invention can effectively prevent and control agricultural insects and especially have an efficient killing function for insect larvae in the overwintering period. The ice nucleus active bacteria compound is capable of lowering the under-cooling point of the insect larvae and freezing the insect larvae to die or hurt under relative higher temperature in the overwintering period. The method disclosed by the invention is simple. After the ice nucleus active bacteria compound is used, no drug resistance is generated, no pollution is caused and the ice nucleus active bacteria compound is long-term effective.

Description

technical field [0001] The invention relates to the field of pest prevention and control of agricultural crops, in particular to a preparation method of an ice nucleation active bacterial composition for pest control. Background technique [0002] Crop pests are an important limiting factor affecting crop yield and income. For example, rapeseed borer harms plants such as fennel, sugar beet, cabbage, rapeseed, shepherd's purse, radish, cabbage, and mustard greens. [0003] For the prevention and control of diseases and insect pests, the prior art generally uses the method of spraying chemical pesticides. This method has significant defects. Although the short-term efficacy is high, the residual toxicity of chemical pesticides is long. After long-term use, the pests will develop resistance. The effect is greatly reduced. [0004] Moreover, the large-scale input and long-term use of chemical pesticides have brought about many problems. Due to the large-scale application of ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P7/04C12N1/20C12R1/38C12R1/18C12R1/64
CPCA01N63/10C12N1/20A01N2300/00
Inventor 屈庆涛屈凡河赵越
Owner 黎昊源生物工程(山东)股份有限公司
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