A detection method for the pathogen of sweet potato seed potato compound virus disease (SPVD)

Abstract

The invention provides a method for detecting the pathogen of sweet potato seed potato compound virus disease (SPVD), comprising: extracting sweet potato seed potato RNA; reversely transcribing the RNA into cDNA; using the cDNA as a template, and carrying out the first round of PCR with designed primers Amplify, and use agarose gel electrophoresis to carry out the first round of detection to the first round of amplified products, wherein, the design primers include outer primers and inner primers; use agarose gel electrophoresis to separate the first round of amplified products, and recover target DNA; and using the recovered target DNA as a template, performing a second round of PCR amplification with internal primers, and performing a second round of detection on the second round of amplification products by agarose gel electrophoresis. The method effectively shortens the detection time, improves the detection accuracy, and is suitable for actual production processes such as sampling detection of sweet potato seeds.

Description

technical field [0001] The invention relates to the technical field of detection of crop diseases and insect pests, in particular to a method for detecting the pathogen of sweet potato seed potato complex virus disease (SPVD). Background technique [0002] Sweet potato virus diseases (SPVD) are caused by the co-infection of sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV). virus disease. During the co-infection process, SPCSV acts as a provoking virus, mediates the synergy between the two, facilitates the replication and transportation of SPFMV in plants, significantly enhances the pathogenicity of SPFMV, and makes the symptoms more obvious. The synergistic effect will promote the movement and accumulation of viruses in plants, affect the yield of storage roots, and aggravate the symptoms of viral diseases, resulting in reduced yield and quality of sweet potatoes, and even severe crop failure. [0003] At present, SPVD is the most h...

AI Technical Summary

Problems solved by technology

At home and abroad, considerable achievements have been made in the propagation of virus-free sweet potato seed potatoes. However, the detection of virus-free effects and the quality control of seed potatoes need to be strengthened

Due to the long time required for phenotypic identification and physiological index detection, the actual production and application are relatively limited. The mainstream rapid detection methods for sweet potato compound virus diseases are mainly enzyme-linked immunoassay and fluorescent quantitative PCR detection. , there are problems such as high requirements for testing equipment, high testing costs, false positives and false negatives, etc., which are not easy to be accepted by the actual production process

Images