A detection method for the pathogen of sweet potato seed potato compound virus disease (SPVD)

A technology of compound virus and detection method, which is applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems that the quality control of seed potato needs to be strengthened, the actual production and application are limited, and the requirements for detection equipment are high. , to achieve the effect of easy promotion and mastery, short detection period and low professional skills requirements
CN107385117BActive Publication Date: 2021-08-10湖南省作物研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
湖南省作物研究所
Publication Date
2021-08-10

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Abstract

The invention provides a method for detecting the pathogen of sweet potato seed potato compound virus disease (SPVD), comprising: extracting sweet potato seed potato RNA; reversely transcribing the RNA into cDNA; using the cDNA as a template, and carrying out the first round of PCR with designed primers Amplify, and use agarose gel electrophoresis to carry out the first round of detection to the first round of amplified products, wherein, the design primers include outer primers and inner primers; use agarose gel electrophoresis to separate the first round of amplified products, and recover target DNA; and using the recovered target DNA as a template, performing a second round of PCR amplification with internal primers, and performing a second round of detection on the second round of amplification products by agarose gel electrophoresis. The method effectively shortens the detection time, improves the detection accuracy, and is suitable for actual production processes such as sampling detection of sweet potato seeds.
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Description

technical field

[0001] The invention relates to the technical field of detection of crop diseases and insect pests, in particular to a method for detecting the pathogen of sweet potato seed potato complex virus disease (SPVD). Background technique

[0002] Sweet potato virus diseases (SPVD) are caused by the co-infection of sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV). virus disease. During the co-infection process, SPCSV acts as a provoking virus, mediates the synergy between the two, facilitates the replication and transportation of SPFMV in plants, significantly enhances the pathogenicity of SPFMV, and makes the symptoms more obvious. The synergistic effect will promote the movement and accumulation of viruses in plants, affect the yield of storage roots, and aggravate the symptoms of viral diseases, resulting in reduced yield and quality of sweet potatoes, and even severe crop failure.

[0003] At present, SPVD is the most h...

Claims

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