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Preparation method of nanometer micro particles embedded with anti-cancer medicine loaded carbon quantum dots

A carbon quantum dot and nanoparticle technology, which is applied in the field of nanoparticle preparation, can solve the problems of unsatisfactory carbon quantum dot loading effect, etc., and achieve the effects of improving the encapsulation rate and simple experimental operation process.

Active Publication Date: 2017-11-28
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the loading effect of carbon quantum dots on drugs is not ideal enough, and it still needs to be further improved. At the same time, how to improve the fluorescence effect of carbon quantum dots also needs to be improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Add 400 mg of citric acid to 20 mL of hexadecylamine to obtain a mixed solution, which is sealed and stored.

[0034] 2) Add the mixture obtained in 1) to 60 mL of concentrated sulfuric acid (98wt%) and 25 mL of concentrated nitric acid (65wt%) mixed acid solution, and the mixture was first ultrasonically dispersed for 1 h, and then stirred at 100 °C Reflux for 24 h. The obtained solution was cooled to room temperature first, and then diluted with deionized water to a total volume of about 600 mL.

[0035] 3) Add sodium carbonate to 2) neutralize the excess acid until the pH of the solution is around 7.5. The obtained solution was concentrated by rotary evaporation, and a large amount of precipitated inorganic salts were removed by filtration. The collected filtrate was dialyzed with a dialysis bag (1000 Da) for 3 days to obtain an aqueous solution of pure carbon quantum dots.

[0036] 4) Add 10 mL of ammonia water and 10 mL of thionyl chloride to 30 mL of the carb...

Embodiment 2

[0046] 1) Add 450 mg of citric acid to 25 mL of hexadecylamine to obtain a mixture, which is sealed and stored.

[0047] 2) Add the mixture obtained in 1) to a mixture of 65 mL of concentrated sulfuric acid (98wt%) and 27 mL of concentrated nitric acid (65wt%). The mixture is first ultrasonically dispersed for 1 h, and then stirred and refluxed at 100°C 27 h. The obtained solution was first cooled to room temperature, and then diluted with deionized water to a total volume of about 700 mL.

[0048] 3) Add sodium carbonate to 2) neutralize the excess acid until the pH of the solution is around 8.0. The obtained solution was concentrated by rotary evaporation, and a large amount of precipitated inorganic salts were removed by filtration. The collected filtrate was dialyzed with a dialysis bag (1000 Da) for 3 days to obtain an aqueous solution of pure carbon quantum dots.

[0049] 4) Add 13 mL of ammonia water and 13 mL of thionyl chloride to 35 mL of the carbon quantum dot sol...

Embodiment 3

[0059] 1) Add 500 mg of citric acid to 30 mL of hexadecylamine to obtain a mixture, which is sealed and stored.

[0060] 2) Add the mixture obtained in 1) to a mixture of 70 mL of concentrated sulfuric acid (98wt%) and 30 mL of concentrated nitric acid (65wt%). The mixture is ultrasonically dispersed for 2 h, and then stirred and refluxed at 100°C 30 h. The obtained solution was first cooled to room temperature, and then diluted with deionized water to a total volume of about 800 mL.

[0061] 3) Add sodium carbonate to 2) neutralize the excess acid until the pH of the solution is around 8.5. The obtained solution was concentrated by rotary evaporation, and a large amount of precipitated inorganic salts were removed by filtration. The collected filtrate was dialyzed with a dialysis bag (1000Da) for 4 days to obtain an aqueous solution of pure carbon quantum dots.

[0062] 4) Add 15 mL of ammonia water and 15 mL of thionyl chloride to 40 mL of the carbon quantum dot solution o...

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Abstract

The invention relates to the field of medicine, and discloses a preparation method of nanometer micro particles embedded with anti-cancer medicine loaded carbon quantum dots. The carbon quantum dots are firstly prepared and synthesized through the effects of citric acid and a mixed solution of concentrated sulfuric acid and concentrated nitric acid, so that anti-cancer medicine of Sorafenib is adsorbed to obtain the medicine loaded carbon quantum dots; then, a secondary emulsification-solvent volatilization method is used for preparing the polymer nanometer particles embedded with the medicine loaded carbon quantum dots. The polymer micro particles prepared by the method are hopeful to realize the bioimaging in the later period and is hopeful to be used for treating cancer cells; meanwhile, the method cannot generate the obvious influence on the cells in the later stage experiment process; the scientificity of the experiment result is not influenced; the experiment operation process is simple, non-toxic and harmless, and achieves the green and environment-friendly effects.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for preparing nanoparticles embedded with carbon quantum dots loaded with anticancer drugs. Background technique [0002] Due to their unique properties, carbon quantum dots have aroused great interest of researchers. Quantum dots have unique optical characteristics: the fluorescence wavelength can be adjusted by changing the particle size and composition, strong fluorescence, good photostability, wide excitation spectrum and narrow emission fluorescence spectrum. With the extensive research of quantum dots, quantum dots have shown many research values: quantum dots have been widely used in biomedical research fields such as biology, cell biosensing, animal living imaging, and tumor marker detection. [0003] The main means of research is to load drugs on quantum dots so that they can not only have biological imaging but also be used for the treatment of cancer cells. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/04A61K31/44A61K9/50A61K49/00C01B32/15A61P35/00
CPCA61K9/5031A61K31/44A61K47/02A61K49/0019
Inventor 王秉梁军龙陈茹茹李津黄芊蔚
Owner ZHEJIANG SCI-TECH UNIV
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