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Preparation method of biomimetic tissue engineering scaffold with photothermal responsiveness and controlled drug release

A tissue engineering scaffold and a responsive technology, applied in the field of bionic tissue engineering scaffold preparation, can solve problems such as accelerated nerve regeneration, achieve the effect of improving the success rate, realizing personalized treatment and application, and facilitating long-term treatment and rehabilitation

Active Publication Date: 2022-05-03
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Purpose of the invention: In view of the existing problems and the deficiencies of the prior art, the technical problem to be solved by the present invention is to construct an artificial nerve graft that contains intelligent responsive controlled release bioactive molecules and has a bionic micro-nano topology on the surface. In order to better realize the function of accelerating nerve regeneration, it provides a beneficial choice for patients with peripheral nerve injury in clinical practice

Method used

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  • Preparation method of biomimetic tissue engineering scaffold with photothermal responsiveness and controlled drug release
  • Preparation method of biomimetic tissue engineering scaffold with photothermal responsiveness and controlled drug release
  • Preparation method of biomimetic tissue engineering scaffold with photothermal responsiveness and controlled drug release

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Experimental program
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Effect test

specific Embodiment 1

[0061] The first step is to prepare a nanoparticle with photothermal effect loaded with bioactive molecules:

[0062] Take tris-tris to prepare a Tris solution with a pH of 8.5, then add Dopa powder to the Tris solution to prepare a polydopamine solution, and finally add 50 mg of nanoparticles to 10 ml of the polydopamine solution. Wrap the container containing the mixed solution with tin foil to avoid light, ventilate it, and shake it in a mechanical shaker. After shaking, remove the solution from the shaker container, discard the supernatant, take out the precipitate and dry it. Then dry the obtained powder and wash it again with Milli-Q. After centrifuging again, the supernatant was discarded, and the precipitate was dried to obtain Dopa@MWCNT powder. Add Dopa@MWCNT powder into 20ml of DFO / YR mixed solution, and shake it in a mechanical shaker. After shaking, the solution was taken out from the shaker and placed in a centrifuge for centrifugation. Finally, discard the s...

specific Embodiment 2

[0070] The first step is to prepare a nanoparticle with photothermal effect loaded with bioactive molecules:

[0071] Take tris-tris to prepare a Tris solution with a pH of 8.5, then add Dopa powder to the Tris solution to prepare a polydopamine solution, and finally add 100 mg of nanoparticles to 10 ml of the polydopamine solution. Wrap the container containing the mixed solution with tin foil to avoid light, ventilate it, and shake it in a mechanical shaker. After shaking, remove the solution from the shaker container, discard the supernatant, take out the precipitate and dry it. Then dry the obtained powder and wash it again with Milli-Q. After centrifuging again, the supernatant was discarded, and the precipitate was dried to obtain Dopa@MWCNT powder. Add Dopa@MWCNT powder into 20ml of DFO / YR mixed solution, and shake it in a mechanical shaker. After shaking, the solution was taken out from the shaker and placed in a centrifuge for centrifugation. Finally, discard the ...

specific Embodiment 3

[0079] The first step is to prepare a nanoparticle with photothermal effect loaded with bioactive molecules:

[0080] Take tris-tris to prepare a Tris solution with a pH of 8.5, then add Dopa powder to the Tris solution to prepare a polydopamine solution, and finally add 150 mg of nanoparticles to 10 ml of the polydopamine solution. Wrap the container containing the mixed solution with tin foil to avoid light, ventilate it, and shake it in a mechanical shaker. After shaking, remove the solution from the shaker container, discard the supernatant, take out the precipitate and dry it. Then dry the obtained powder and wash it again with Milli-Q. After centrifuging again, the supernatant was discarded, and the precipitate was dried to obtain Dopa@MWCNT powder. Add Dopa@MWCNT powder into 20ml of DFO / YR mixed solution, and shake it in a mechanical shaker. After shaking, the solution was taken out from the shaker and placed in a centrifuge for centrifugation. Finally, discard the ...

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Abstract

The invention discloses a preparation method of a biomimetic tissue engineering scaffold with photothermal responsiveness and controllable drug release. The scaffold contains bioactive molecules that can promote nerve regeneration and can be released under photocontrol, and the surface of the scaffold has the ability to regulate cell orientation The anisotropic topological structure of growth can better mimic the microenvironment of nerve regeneration. The specific steps are as follows: preparing biologically active nanoparticles; preparing an electrospinning receiving substrate; preparing a polymer biomaterial solution, blending and electrospinning the bioactive nanoparticle and the polymer biomaterial solution; peeling off the scaffold to obtain the scaffold. The stent of the present invention has excellent biocompatibility, mechanical properties, intelligent responsiveness and controlled drug release performance, can accelerate nerve regeneration, and solve the problems of unsatisfactory repair effect of long-distance nerve defects and lack of functional recovery. Ideal materials and preparation methods are provided for patients with nerve injury with poor nerve recovery.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of a bionic tissue engineering scaffold with photothermal responsiveness and controllable drug release. Background technique [0002] Peripheral nerve injury is a common clinical disease. For those with short defect gaps, precision suture or small gap cannula can be used to directly repair the defect, but for long-distance nerve injuries, direct suture is not feasible. At present, with the development of microsurgical equipment and technology, the clinical treatment effect of peripheral nerve injury has been continuously improved. More methods are used to bridge tissue engineering grafts, which has been proved to be an effective method for repairing peripheral nerve injury. However, Compared with autologous nerve grafts, there is still a gap in its repair effect. Therefore, it is urgent to develop new tissue engineered nerve grafts. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/50A61L27/58A61L27/22A61L27/26A61L27/08D01D5/00D04H1/4382D04H1/728
CPCA61L27/54A61L27/50A61L27/58A61L27/227A61L27/08A61L27/26D04H1/4382D04H1/728D01D5/003D01D5/0092D01D5/0069D01D5/0061A61L2300/624A61L2430/32C08L5/08C08L67/04
Inventor 李贵才刘逸凡慕函朔刘毅恒韩琦梁佳琦张林辉刘恒全张鲁中杨宇民
Owner NANTONG UNIVERSITY
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