Device and method for detection of haemoglobin and its complexes

A technology of hemoglobin and complexes, applied in the field of biosensors for biological analytes, can solve problems such as the difficulty of direct electrochemical detection of hemoglobin

Active Publication Date: 2017-11-28
INDIAN INSTITUTE OF SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct electrochemical detection of hemoglobin is difficult

Method used

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  • Device and method for detection of haemoglobin and its complexes
  • Device and method for detection of haemoglobin and its complexes
  • Device and method for detection of haemoglobin and its complexes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0226] Example 1: Using pyridine and NaOH as receptors to measure blood hemoglobin concentration and corresponding reduction current

[0227] 1.5ml whole blood was lysed with 4ml cold deionized (DI) water. 2ml of 1% NaOH was added to the lysis solution to convert the hemoglobin component into methemoglobin. 1.5 ml of pyridine was added to the methemoglobin solution to convert it into pyridine methemochromogen. From this master solution, hemoglobin solutions of different concentrations are prepared by appropriately diluting the pyridine methemochromogen solution. A final volume of 300 μL was used for testing.

[0228] Obtain the required volume of blood sample and distribute it on the electrodes of the biosensor device, and obtain the corresponding cyclic voltammogram by using the value of the CHI electrochemical workstation. The potential window used varies from 0.4V to -1.2V, The scan rate is 0.6V / sec, as shown in Figure 21(a).

[0229] The cold DI water lyses the RBC in the blo...

Embodiment 2

[0236] Example 2: Measurement of hemoglobin using pyridine and NaOH receptors

[0237] A 300 μL sample volume of pyridine methemochromogen was placed on the electrode, and then the peak reduction current value was recorded from the cyclic voltammogram with a potential window of 0.4V to -1.2V in the CHI electrochemical workstation. The measured value of the peak reduction current is 238 μA. Find the current value in Table 1, and retrieve the corresponding hemoglobin concentration of 2.4g / dL.

Embodiment 3

[0238] Example 3: Using pyridine and NaOH as receptors to determine the blood hemoglobin concentration in the physiological range and the corresponding reduction current

[0239] 1.5ml whole blood was lysed with 4ml cold deionized water. 2ml of 1% NaOH was added to the lysis solution to convert the hemoglobin component into methemoglobin. Solid bovine hematin is added to the solution to further increase the methemoglobin content in the physiological range. Then 1.5ml of pyridine was added to the methemoglobin solution to convert it into pyridine methemochromogen. From this master solution, hemoglobin solutions of different concentrations are prepared by appropriately diluting the pyridine methemochromogen solution. A final volume of 300 μL was used for testing.

[0240] Obtain the required volume of blood sample and distribute it on the electrodes of the biosensor device, and obtain the corresponding cyclic voltammogram by using the value of the CHI electrochemical workstation. ...

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Abstract

An electrochemically active device is provided for collecting and retaining a blood sample with at least a two-electrode member connected to conductive tracks. A receptor with an integral receptor-membrane arranged on the two-electrode member, to receive non-electrochemically active heamoglobin bioanalyte and its complexes from red blood cells (RBC) of said blood sample, through a lysing agent and convert the non-electrochemically active heamoglobin bioanalyte and its complexes, into an electrochemically active bioanalyte and its electrochemically active complexes. The present invention also provides a point-of-care biosensor incorporated with the device of the present invention and method of measuring for the detection and quantitative measurement of concentrations of haemoglobin (Hb), glycated haemoglobin (GHb), methaemoglobin (MetHb) and myoglobin, in reduced volumes of blood samples, by determining redox current values in the reduced volumes of blood samples.

Description

Technical field [0001] The present invention generally relates to a biosensor and method for quantitatively measuring biological analytes in biological samples. More specifically, the present invention relates to a method for converting non-electrochemically active hemoglobin biological analytes and complexes in a reduced volume of blood sample into electrochemically active biological analytes and complexes for accurate detection and quantitative measurement of hemoglobin, Electrochemically active biosensor for glycosylated hemoglobin (GHb), methemoglobin and myoglobin. Background technique [0002] Oxygen is the most versatile reagent used in nature for many different functions in the human body. Compared with polar solvents, oxygen is more soluble in non-polar solvents. Simple diffusion cannot deliver oxygen quickly enough to internal cells in multicellular organisms; therefore, carriers such as hemoglobin and myoglobin are required. Hemoglobin is the oxygen carrier protein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/1468
CPCA61B5/726A61B5/1477A61B2562/0295G01N27/4168G01N33/721
Inventor 维纳伊·库马尔纳瓦坎塔·巴特
Owner INDIAN INSTITUTE OF SCIENCE
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