Bioreactor for online automatic pH control and control method thereof

[0023] The following describes the implementation of the present invention in detail with reference to the drawings and embodiments.

[0024] Such as figure 1 , A bioreactor with online automatic pH regulation, including data acquisition controller 1, alkali storage tank 2, acid storage tank 3, debugging tank 4, and biological reaction tank 5. The volume range of debugging tank 4 is 0.020-20L, and the biological reaction tank 5 can be an anaerobic biological reactor or an aerobic biological reactor. Among them, the data acquisition controller 1 includes a pH acquisition unit 11, a liquid level acquisition unit 12, and a solenoid valve and peristaltic pump control unit 13. The alkali storage tank 2 is connected to the debugging tank 4 through a pipeline with a solenoid valve 21. The pipeline of valve two 22 is connected to the bioreactor 5. The acid storage tank 3 is connected to the debugging tank 4 through a pipeline with a solenoid valve three 31, and is connected to the biological reaction tank 5 through a pipeline with a solenoid valve four 32. The liquid inlet of the debugging tank 4 is connected to the bioreactor 5 through a pipeline with a peristaltic pump 45, and the liquid outlet is connected to the biological reaction tank 5 through a pipeline with a solenoid valve 46. The debugging tank 4 is equipped with a stirrer 1. 42. The pH meter 43 and the liquid level meter 44. The bioreactor 5 is provided with an air outlet 51, a stirrer 2 52 and a liquid level meter 53. The pH meter 43 is connected to the pH collection unit 11, and the liquid level meter 44 is connected to the liquid level meter. The position gauge two 53 is connected to the liquid level acquisition unit 12, the solenoid valve one 21, the solenoid valve two 22, the solenoid valve three 31, the solenoid valve four 32, the solenoid valve five 46, and the peristaltic pump 45 are connected to the solenoid valve and peristaltic pump control unit 13 .

[0025] Further, the bottom of the pipeline connecting the alkali storage tank 2 and the acid storage tank 3 to the bioreactor 5 is provided with a microporous water distributor 23.

[0026] Further, under anaerobic conditions, the structure type of the bioreactor 5 can be a completely mixed anaerobic reactor (CSTR), an anaerobic membrane bioreactor (AnMBR), an upflow anaerobic sludge bed (UASB) reaction Reactor or other anaerobic bioreactor; under aerobic conditions, the structure of the bioreactor 5 can be an airlift internal loop reactor (ILAR), a sequencing batch reactor (SBR), and a high-efficiency aerobic bioreactor (HCR) or other aerobic bioreactors.

[0027] Further, if the biological reaction tank 5 is an anaerobic biological reaction tank, a breathing bag 41 is provided on the debugging tank 4, and the maximum expansion volume of the breathing bag 41 is the same as the volume of the debugging tank 4; if the biological reaction tank 5 is aerobic For the biological reaction tank, the breathing bag 41 is not provided on the debugging tank 4.

[0028] Further, the regulation method of the above-mentioned on-line automatic pH regulation bioreactor is as follows:

[0029] Input the pH control target value through the man-machine interface of the data acquisition controller 1 obj; Set a fixed interval time t, the solenoid valve and the peristaltic pump control unit 13 turn on the peristaltic pump 45 to pump the reaction liquid in the bioreactor 5 into the debugging tank 4, and read the volume V of the reaction liquid through a level gauge 44 1;

[0030] If the measured value of pH meter 43 is pH mt Equal to pH obj , Without adding acid and alkali reagents, directly open the solenoid valve No. 46, and reinject the reaction liquid into the bioreactor 5;

[0031] If pH mt Less than pH obj , Open solenoid valve 21 intermittently, dosing alkaline reagents intermittently until pH mt Equal to pH obj , Stop dosing, read the volume V through the level gauge 44 2 , Read the volume V of the reaction liquid in the bioreactor 5 through the level gauge 2 53 3 , Calculate and adjust bioreactor 5 to pH obj The volume of alkali reagent needed to be added is (V 2 -V 1 )V 3 /V 1;

[0032] If pH mt Greater than pH obj , Open solenoid valve three 31 intermittently, and add acid reagents intermittently until pH mt Equal to pH obj , Stop dosing, read the volume V through the level gauge 44 2 , Read the volume V of the reaction liquid in the bioreactor 5 through the level gauge 2 53 3 , Calculate and adjust bioreactor 5 to pH obj The volume of acid reagent needed to be added is (V 2 -V 1 )V 3 /V 1;

[0033] According to the calculation result, open the solenoid valve 22 and solenoid valve 432, add alkali or acid reagent to the bioreactor 5, after the pH control is completed, open the solenoid valve 546, and reinject the adjusted liquid in the debugging tank 4 into the biological reactor 5.

[0034] When the pH adjustment is completed, the pollutant concentration C in the bioreactor 5 0 Down to kC 0; Among them, k is the concentration correction coefficient, k=V 1 /V 2.