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Use of Chinese patent medicine Kongsial pills in the preparation of anti-HIV latent drugs

A technology of Chinese patent medicine and medicine, applied in the field of medicine

Active Publication Date: 2021-03-19
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been no report about the anti-HIV latent therapeutic effect of Kongsial Pills.

Method used

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  • Use of Chinese patent medicine Kongsial pills in the preparation of anti-HIV latent drugs
  • Use of Chinese patent medicine Kongsial pills in the preparation of anti-HIV latent drugs
  • Use of Chinese patent medicine Kongsial pills in the preparation of anti-HIV latent drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Controlling pills induce expression of latent HIV-1 on C11 cells

[0038] 2×10 per hole 4 C11 cells were inoculated in a 96-well plate, and 100 μL of 1640 medium (Gibco) containing 10% FBS (Gibco) was added to each well, and a final concentration of 25 μg / mL of salivary control was added according to the ratio of drug to medium 1:200. Pills; after drug treatment for 72 h, the expression of GFP in the cells was observed under a fluorescence microscope, and the cells were collected for flow cytometry detection, and the proportion of fluorescent cells was analyzed;

[0039] The results showed that the Kongsial Pills could induce the expression of latent HIV-1; HIV-1 latently infected cells C11 had a green fluorescent positive cell ratio as high as 66.2% after being treated with the Kongsial Pills, while the HIV-1 cells that were not treated with the inducer For latently infected cells, the proportion of fluorescent positive cells was only 3.80% background activ...

Embodiment 2

[0040] Example 2 The expression of latent HIV-1 induced by Kongsial pills has a dose-dependent effect,

[0041] 2×10 per hole 4 C11 cells were planted in a 96-well plate, 100 μL of 1640 medium (Gibco) containing 10% FBS (Gibco) was added to each well, and different concentrations of control salivary pills were added according to the ratio of drug to medium 1:200; drug treatment After 72 h, the cells were collected for flow cytometry detection, and the proportion of fluorescent cells was analyzed;

[0042] The results showed that after HIV-1 latently infected cells C11 were treated with Kongsial pills, the number of cells expressing green fluorescence increased as the concentration of Kongsial pills increased; when the concentration of Kongsial pills increased to 250 When μg / mL, the proportion of GFP positive cells increased from 5.01% to 70.40%; while HIV-1 latently infected cells without inducer treatment, the proportion of fluorescent positive cells was only 3.80% backgroun...

Embodiment 3

[0043] Example 3 Controlling pills combined with antiretroviral drugs and other immune preparations or modified cells

[0044] Further adopt the control salivary pill treatment plan of embodiment 1 or embodiment 2 in combination with antiretroviral drugs and other immune preparations or modified cells to observe the impact on HIV-1 latently infected cells. The results show that the control Saliva pills have the effect of inducing the activation of HIV latent cells or interfering with HIV latency. Used in combination with antiretroviral drugs and other immune agents or modified cells, it can kill activated latently infected cells and accelerate the removal of latent virus storage. , and will further provide a new intervention approach for the complete cure of AIDS.

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Abstract

The invention belongs to the field of medicine, and relates to a drug new application of a Chinese patent medicine saliva-controlling pellet, in particular to an application of the Chinese patent medicine saliva-controlling pellet for preparing an anti-HIV latent drug. The approval number of the saliva-controlling pellet is State Food and Drug Administration approval number Z11021138. The experimental result proves that the saliva-controlling pellet has a high inducing and activating effect on a latent infection cell model, the activating effect of the saliva-controlling pellet has a dosage dependent effect, and the anti-HIV latent drug can be prepared; and a drug combination can be further prepared from the various anti-HIV drugs of the saliva-controlling pellet and a nucleoside reverse transcriptase inhibitor or non-nucleoside reverse transcriptase inhibitor or protease inhibitor and the like, the drug composition is used for activating and removing latent virus, and finally a virus reservoir is removed or reduced. The application of the Chinese patent medicine saliva-controlling pellet for preparing the anti-HIV latent drug provides a new path and means for inducing, activating and finally removing HIV latent infection virus.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a new drug application of the Chinese patent drug Kongsial pills, in particular to the application of the Chinese patent drug Kongsial pills in preparing anti-HIV latent drugs. Background technique [0002] AIDS, or Acquired Immunodeficiency Syndrome (AIDS), is an infectious disease caused by human immunodeficiency virus (Human Immunodeficiency Virus, HIV-1) infection of the immune system, which seriously affects human physical and mental health. In 2012, about 35.3 million people were infected with HIV-1 in the world, of which 2.3 million were newly infected and 1.6 million died. AIDS has become a serious public health and social problem in the world today. At present, the clinical treatment of AIDS is mainly High Active Antiretroviral Treatment (HAART), which can reduce the plasma HIV-1 level below the clinical detection line, greatly improve the quality of life of patients, and enable pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/74A61K9/20A61K45/06A61K39/395A61P31/16A61K35/17A61K35/28
CPCA61K9/2004A61K35/17A61K35/28A61K36/31A61K36/47A61K36/74A61K39/395A61K45/06A61K2300/00
Inventor 朱焕章陆盼盼
Owner FUDAN UNIV