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Almond anther intercellular polysaccharide granule dying method

A dyeing method, the technology of almond blossom, applied in the preparation of test samples, sampling, measuring devices, etc., can solve the problem of not being able to distinguish polysaccharide particles well

Inactive Publication Date: 2017-12-12
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For traditional I 2 -KI staining method can stain polysaccharide particles blue, but when observing polysaccharide particles with a microscope, the light is irradiated from bottom to top, and the imaging system cannot distinguish polysaccharide particles well

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] (1) Fix the material, prepare FAA fixative solution (glacial acetic acid: 38% formaldehyde: 70% ethanol = 5:5:90), fix anthers of different periods in FAA fixative solution, replace the fixative solution after the 7th day, and After 14 days, the fixative was changed again, and then the fixative and the samples were stored in a refrigerator at 4°C. (2) Solution preparation, respectively prepare 0.5% periodic acid solution, 1N hydrochloric acid, colorless magenta solution (Schiff's solution) and 0.5% sodium metabisulfite solution. The preparation method of 0.8% periodic acid solution is to add 0.8 g of periodic acid to 100 ml of distilled water; the preparation method of 1N hydrochloric acid is to add 8.5 mL of concentrated hydrochloric acid (content 22%) to 91.5 mL of distilled water; the colorless fuchsin solution (Schiff's solution) is prepared by adding 0.8 g of basic fuchsin to 100 mL of boiled distilled water, fully dissolving for 10 min, cooling to 45-50 °C and fil...

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PUM

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Abstract

The invention relates to an almond anther intercellular polysaccharide granule dying method. The almond anther intercellular polysaccharide granule dying method comprises dying almond anther cell sections for 0.5 h through prepared dye liquor, absorbing superfluous dye liquor through filter paper, performing color separation through 0.5% sodium metabisulfite solution and color washing through distilled water, air-drying the cell sections and placing the cell sections under a microscope for observation of polysaccharide granules or immobilizing the cell sections in neutral balsam into permanent sections. When observed under the microscope, polysaccharide granules inside almond anther cells are dyed into bright red or deep red, and the higher the content of the polysaccharide granules is, the darker the dyed color is. The almond anther intercellular polysaccharide granule dying method can help observe distribution of the polysaccharide granules in almond anther epidermal cells, endothecium cells, intermediate cells, tapetum cells, pollen grains and conjunctive tissues and can be applied to research on the effects of the polysaccharide granules on growth on pollens and cracking and glycometabolism of anthers.

Description

technical field [0001] The invention patents a method for staining polysaccharide particles in almond anther cells. Under the condition of avoiding light, use the dye solution prepared in advance to stain the slices of almond anther cells for 0.5 h, first absorb the excess dye solution with filter paper, and then use 0.5 % sodium bisulfite solution for color separation and distilled water for color washing, after drying the slices, put the slices under a microscope to observe the polysaccharide particles, or make permanent slices after being fixed with neutral gum. Observation under the microscope found that the polysaccharide particles in the anther cells of almond peach were dyed bright red or deep red, and the more polysaccharide particles contained, the darker the staining. The invention method of this patent can be used to observe the distribution of polysaccharide particles in tonsil anther epidermal cells, inner wall cells, middle layer cells, tapetum cells, pollen grai...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/305
Inventor 李疆李鹏田嘉罗淑萍王磊李长江张琦
Owner XINJIANG AGRI UNIV