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SNP molecular marker interlocked with flowering Chinese cabbage anthocyanin gene and application of SNP molecular marker

A technology of cabbage anthocyanins and molecular markers, applied in the field of molecular detection, can solve the problems of time-consuming and labor-intensive, unfavorable cabbage breeding, etc., and achieve the effect of shortening the time

Active Publication Date: 2017-12-15
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Although it is feasible to breed high anthocyanin varieties by traditional breeding methods, it is time-consuming and labor-intensive, which is not conducive to the breeding of Chinese cabbage sprouts

Method used

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  • SNP molecular marker interlocked with flowering Chinese cabbage anthocyanin gene and application of SNP molecular marker
  • SNP molecular marker interlocked with flowering Chinese cabbage anthocyanin gene and application of SNP molecular marker
  • SNP molecular marker interlocked with flowering Chinese cabbage anthocyanin gene and application of SNP molecular marker

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Experimental program
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Effect test

Embodiment 1

[0037] 1. Genetic population construction and genetic analysis

[0038] 1. Plant materials for the test: the material with high anthocyanin content is a high-generation inbred line obtained from Hunan Hongsai No. Nong 50 Tian Caixin is a high-generation inbred line obtained by multi-generation selfing. figure 1 It is the plant photos of P1 and P2, the female parent (P1) with high anthocyanin content, the cabbage color is purple red; and the male parent (P2) with low anthocyanin content, the cabbage color is green. F1 was obtained by crossing Xianghongsai No. 1 and Yinong 50-day Caixin. F1 was self-crossed to obtain F2, and BC1 was obtained by backcrossing at the same time. The color of each generation was observed, and the color separation ratio of the segregation generations was counted. Genetic analysis was carried out, and the purple-red Traits are positioned.

[0039] 2. Analysis of genetic law of anthocyanins in Chinese cabbage sprouts

[0040]The statistical results o...

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Abstract

The invention discloses an SNP molecular marker interlocked with a flowering Chinese cabbage anthocyanin gene, and application of the SNP molecular marker. The molecular marker 2276542 and the molecular marker 2385613 obtained by screening in the invention are tightly interlocked with the flowering Chinese cabbage anthocyanin gene; the physical distance between the two markers is 0.336 Mb; the molecular marker can be directly used for establishing a flowering Chinese cabbage anthocyanin gene molecular marker auxiliary breeding system. A dCAPS amplification primer designed according to the two molecular markers can be conveniently and quickly applied to flowering Chinese cabbage variety improvement molecule assistant breeding with high throughput, thereby providing the technical support for flowering Chinese cabbage appearance quality molecule breeding, and meanwhile, greatly shortening the conventional gene positioning time.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a SNP molecular marker linked with a cabbage color gene and an application thereof. Background technique [0002] Anthocyanins, also known as anthocyanins, belong to bioflavonoid compounds in phenolic compounds. They are widely present in plants and are one of the pigments that are easily soluble in water. Anthocyanins constitute the components of fruits, vegetables, flowers and other plants. Colorful colors, in the natural state, the anthocyanins present in the vacuole are substances formed by combining anthocyanin aglycone with different types of monosaccharides. Anthocyanin is a strong oxidant. The anthocyanin in vegetables not only endows them with colorful colors, increases people's appetite, but also has good health care functions. Studies have shown that anthocyanins have extremely significant effects in anti-oxidation, anti-mutation, protection of...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 钟玉娟黄河勋罗少波李俊星周洋洋谢大森
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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