A kind of assay method of Oxytropis verticillum
A technology of Oxytropis verticillum and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as quality inspection and complex chemical components of Oxytropis verticillum, and achieve accurate results, strong specificity, and reproducibility Good results
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Embodiment 1
[0023] Take 0.3g of the medicinal material to be tested, grind it finely, pass through a 80-mesh sieve, add 30mL of methanol for ultrasonic treatment, filter, evaporate the filtrate to dry methanol, dissolve it with absolute ethanol, extract the dissolved solution with ethyl acetate, concentrate to dryness, and use Dissolve in absolute ethanol to 1 mL, as the test solution, take another 0.3 g of the reference drug, prepare the Oxytropis verticillium control drug solution with the same preparation method as the test solution, and absorb 10 μL of the test solution and the control drug solution Spot on the same silica gel G thin-layer plate with sodium carboxymethyl cellulose as the binder, and use petroleum ether-cyclohexane-toluene-ethyl acetate (volume ratio: 5:15:11:8) as the expansion agent, unfolded, taken out, dried, sprayed with a 12% ethanol solution of sulfuric acid by volume percentage, the wind blows until the spots are clearly colored, and in the sample chromatogram, ...
Embodiment 2
[0025] Take 0.2g of the medicinal material to be tested, grind it finely, pass through a 80-mesh sieve, add 20mL of methanol for ultrasonic treatment, filter, evaporate the filtrate to dry methanol, dissolve it with absolute ethanol, extract the dissolved solution with ethyl acetate, concentrate to dryness, and use Dissolve in absolute ethanol to 1 mL, as the test solution, take another 0.2 g of the reference drug, prepare the Oxytropis verticillium control drug solution with the same preparation method as the test solution, and absorb 12 μL each of the test solution and the reference drug solution Spot on the same silica gel G thin-layer plate with sodium carboxymethyl cellulose as binder, and use petroleum ether-cyclohexane-toluene-ethyl acetate (volume ratio: 4:16:10:6) as the development agent, unfolded, taken out, dried, sprayed with 10% by volume ethanol solution of sulfuric acid, and blown by the wind until the spots were clearly colored, and in the sample chromatogram, ...
Embodiment 3
[0027] Take 0.5g of the medicinal material to be tested, grind it finely, pass through a 80-mesh sieve, add 50mL of methanol for ultrasonic treatment, filter, evaporate the filtrate to dry methanol, dissolve it with absolute ethanol, extract the dissolved solution with ethyl acetate, concentrate to dryness, and use Dissolve in absolute ethanol to 2 mL, as the test solution, take another 0.5 g of the reference drug, prepare the Oxytropis verticillium control drug solution with the same preparation method as the test solution, and absorb 15 μL each of the test solution and the reference drug solution Spot on the same silica gel G thin-layer plate with sodium carboxymethyl cellulose as the binder, and use petroleum ether-cyclohexane-toluene-ethyl acetate (volume ratio: 7:20:15:9) as the development agent, unfolded, taken out, dried, sprayed with a 15% ethanol solution of sulfuric acid by volume, and blown by the wind until the spots were clearly colored, and in the sample chromato...
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