Colloidal gold immunochromatographic test strip for detecting anti-RVF virus antibodies in human and animal serum

An immunochromatographic test strip, colloidal gold technology, applied in immunoassays, biological material analysis, measurement devices, etc., can solve the problems of high investment cost and false positives, and achieve the effects of low cost, strong specificity and convenient use.

Inactive Publication Date: 2017-12-15
GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PCR technology has the advantages of high detection sensitivity, simple operation, and fast speed. However, its initial investment cost is relatively large (depending on laboratory functional partitions and PCR instruments), and it is prone to false positives caused by laboratory contamination. In a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A colloidal gold immunochromatographic test strip for detecting human and animal serum anti-RVF virus antibodies, the test strip contains: a glass fiber membrane of colloidal gold-labeled RVF virus recombinant antigen, coated with specific RVF virus recombinant antigen The detection line of the test line and the nitrocellulose membrane coated with the quality control line of the mouse anti-RVF virus recombinant antigen monoclonal antibody; The corresponding specific RVF virus recombinant antigen reaction on the cellulose membrane forms a specific RVF virus recombinant antigen-specific RVF virus antibody-colloidal gold-labeled RVF virus recombinant antigen sandwich complex, which is judged by the unique color tracer of colloidal gold particles The presence or absence of specific antibodies.

[0031] Wherein, the preparation method of the specific RVF virus recombinant antigen is:

[0032] (1) From the physiological samples or tissue samples of animals infected with RVF ...

Embodiment 2

[0047] A colloidal gold immunochromatographic test strip for detecting human and animal serum anti-RVF virus antibodies, the test strip contains: a glass fiber membrane of colloidal gold-labeled RVF virus recombinant antigen, coated with specific RVF virus recombinant antigen The detection line of the test line and the nitrocellulose membrane coated with the quality control line of the mouse anti-RVF virus recombinant antigen monoclonal antibody; The corresponding specific RVF virus recombinant antigen reaction on the cellulose membrane forms a specific RVF virus recombinant antigen-specific RVF virus antibody-colloidal gold-labeled RVF virus recombinant antigen sandwich complex, which is judged by the unique color tracer of colloidal gold particles The presence or absence of specific antibodies.

[0048] Wherein, the preparation method of the specific RVF virus recombinant antigen is:

[0049] (4) From the physiological samples or tissue samples of animals infected with RVF ...

Embodiment 3

[0064] A colloidal gold immunochromatographic test strip for detecting human and animal serum anti-RVF virus antibodies, the test strip contains: a glass fiber membrane of colloidal gold-labeled RVF virus recombinant antigen, coated with specific RVF virus recombinant antigen The detection line of the test line and the nitrocellulose membrane coated with the quality control line of the mouse anti-RVF virus recombinant antigen monoclonal antibody; The corresponding specific RVF virus recombinant antigen reaction on the cellulose membrane forms a specific RVF virus recombinant antigen-specific RVF virus antibody-colloidal gold-labeled RVF virus recombinant antigen sandwich complex, which is judged by the unique color tracer of colloidal gold particles The presence or absence of specific antibodies.

[0065] Wherein, the preparation method of the specific RVF virus recombinant antigen is:

[0066] (7) From the physiological samples or tissue samples of animals infected with RVF ...

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PUM

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Abstract

The invention discloses a colloidal gold immunochromatographic test strip for detecting anti-RVF virus antibodies in human and animal serum, belonging to the technical field of biological detection. The test strip contains a colloidal gold-labeled specific RVF virus recombinant antigen glass fiber membrane, and a nitrocellulose membrane coated with a specific RVF virus recombinant antigen detection line and coated with a murine anti-RVF virus recombinant antigen monoclonal antibody quality control line. The colloidal gold immunochromatographic test strip disclosed by the invention not only has the advantages of easiness, convenience and quickness, high specificity, high sensitivity, accuracy and reliability, low cost and the like, but also can be used for reading results with naked eyes directly without special instruments, thus having very important medical value for the diagnosis, prevention and treatment of Rift Valley fever virus.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a colloidal gold immunochromatographic test strip for detecting human and animal serum anti-RVF virus antibodies. Background technique [0002] Rift Valley fever is an acute fever Zoonotic natural foci of infectious diseases, mainly through mosquito bites among animals (cattle, sheep, camels, goat etc.) are popular and can also be made by mosquito bites or exposure to Infect animal blood , body fluid or device official (care, slaughter of infected animals, ingestion of raw milk, etc.) and spread to humans. The pathogenic Rift Valley Fever virus (Rift Valley Fevervirus, RVF virus) belongs to the genus Phleboviridae of the family Bunyaviridae. 2-6 days after a person is infected with the virus, flu-like symptoms suddenly appear, with fever, Headache , muscle pain, back pain , some patients develop stiff neck, photophobia, and Vomit , in the early...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577G01N33/558G01N33/56983G01N2333/175G01N2469/20
Inventor 余福勋聂瑛洁罗振华蒋立黄学勇许汴利
Owner GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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