Colloidal gold immunochromatographic test strip for detecting anti-RVF virus antibodies in human and animal serum

Abstract

The invention discloses a colloidal gold immunochromatographic test strip for detecting anti-RVF virus antibodies in human and animal serum, belonging to the technical field of biological detection. The test strip contains a colloidal gold-labeled specific RVF virus recombinant antigen glass fiber membrane, and a nitrocellulose membrane coated with a specific RVF virus recombinant antigen detection line and coated with a murine anti-RVF virus recombinant antigen monoclonal antibody quality control line. The colloidal gold immunochromatographic test strip disclosed by the invention not only has the advantages of easiness, convenience and quickness, high specificity, high sensitivity, accuracy and reliability, low cost and the like, but also can be used for reading results with naked eyes directly without special instruments, thus having very important medical value for the diagnosis, prevention and treatment of Rift Valley fever virus.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a colloidal gold immunochromatographic test strip for detecting human and animal serum anti-RVF virus antibodies. Background technique [0002] Rift Valley fever is an acute fever Zoonotic natural foci of infectious diseases, mainly through mosquito bites among animals (cattle, sheep, camels, goat etc.) are popular and can also be made by mosquito bites or exposure to Infect animal blood , body fluid or device official (care, slaughter of infected animals, ingestion of raw milk, etc.) and spread to humans. The pathogenic Rift Valley Fever virus (Rift Valley Fevervirus, RVF virus) belongs to the genus Phleboviridae of the family Bunyaviridae. 2-6 days after a person is infected with the virus, flu-like symptoms suddenly appear, with fever, Headache , muscle pain, back pain , some patients develop stiff neck, photophobia, and Vomit , in the early...

AI Technical Summary

Problems solved by technology

PCR technology has the advantages of high detection sensitivity, simple operation, and fast speed. However, its initial investment cost is relatively large (depending on laboratory functional partitions and PCR instruments), and it is prone to false positives caused by laboratory contamination. In addition, RVF virus It is a zoonotic natural foci disease, which mostly occurs in areas with relatively poor medical and health conditions in Africa and Asia. Therefore, it is urgent to establish a fast and reliable RVF virus detection technology suitable for grassroots units and field applications.