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Application of protosappanin A derivative in protecting cardiac trauma caused by chemotherapeutics

A chemotherapeutic drug, the technology of hematoxylin, which is applied in the field of medicine, can solve the problems of low output, heavy workload, and difficult operation, and achieve the effects of small side effects, heart protection, and improved quality of life

Active Publication Date: 2017-12-22
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of natural extraction of prohematoxylin A is limited by difficult operation, large workload and low yield, so its application in scientific research and clinical practice has also become a difficulty

Method used

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  • Application of protosappanin A derivative in protecting cardiac trauma caused by chemotherapeutics
  • Application of protosappanin A derivative in protecting cardiac trauma caused by chemotherapeutics
  • Application of protosappanin A derivative in protecting cardiac trauma caused by chemotherapeutics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The improvement effect of 1-(2',4,4',5-tetramethoxy-2-biphenyl)acetone on cardiac function of doxorubicin-treated rats

[0034] experimental method:

[0035] 1. Establishment of adriamycin-induced myocardial injury model in rats

[0036] After doxorubicin was diluted with physiological saline to a concentration of 1 mg / ml, rats were intraperitoneally injected with doxorubicin at a dose of 2.5 mg / kg / w, and a myocardial injury model was obtained after 6 weeks.

[0037] 2. Grouping and administration of animals

[0038] The model animals were given different drugs by intragastric administration, and were randomly divided into three groups: (1) control group, intragastric administration of normal saline; (2) low-dose compound group: given 1-(2',4,4',5-tetramethyl Oxygen-2-biphenyl) acetone (5mg / kg / w) orally; (3) compound high dose group: given 1-(2',4,4',5-tetramethoxy-2- Phenyl) acetone (25mg / kg / w) orally. Animals in the above groups were administered the day...

Embodiment 2

[0045] Example 2 Effect of 1-(2',4,4',5-tetramethoxy-2-biphenyl)acetone on myocardial injury and fibrosis in rats

[0046] The establishment of the rat doxorubicin-induced myocardial injury model, animal grouping and administration are the same as in Example 1.

[0047] Detection indicators and methods:

[0048] 1. Detection of cardiac enzymes and troponin: Immediately after 6 weeks of administration, the rats in the three groups were treated, and the eyeballs were removed to take blood. The operation method and steps were carried out according to the instructions of the cardiac enzyme and troponin kit.

[0049] 2. Masson staining: The experimental procedure is dewaxing of paraffin sections, staining with hematoxylin, differentiation with phosphomolybdic acid aqueous solution for 3-5 minutes, soaking in aqueous glacial acetic acid solution, dehydration, transparent with xylene, and sealing with neutral resin.

[0050] Detection of type III collagen: the rats were treated imme...

Embodiment 3

[0054] Example 3 1-(2',4,4',5-tetramethoxy-2-biphenyl)acetone inhibits cardiomyocyte apoptosis induced by doxorubicin

[0055] experimental method:

[0056] (1) Myocardium cell culture: take the apex of the heart, wash it three times with pre-cooled D-Hank's solution, cut the heart into about 1 cubic millimeter; treat the myocardial tissue with trypsin (0.0625%), and the temperature is 37 ℃, carried out in batches, the time is 5min×5 times, add another 1 minute, repeat 5 times, and so on, until the addition reaches 8 minutes, after the first digestion, the supernatant is naturally precipitated, and after each subsequent natural precipitation, leave Take the supernatant; add the collected supernatant to the same volume of DMEM culture medium, pipette and mix well, the action should be gentle, and centrifuge at 1000r / min for 10min, repeat 3 times. The cells were divided into three groups, ①Adriamycin treatment group: ADR was added, the final concentration was 8 μmol / L ②Compound...

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Abstract

The invention discloses application of a protosappanin A derivative in protecting cardiac trauma caused by chemotherapeutics. A chemical formula of the protosappanin A derivative is as shown in general formula I. Action and a mechanism of protecting cardiac trauma, induced by chemotherapeutics adriamycin, by the protosappanin A derivative are researched; research results show that the protosappanin A derivative can relieve cardiac trauma as well as myocardium cell apoptosis and oxidative stress injury induced by the adriamycin, and can be used as a heart protective drug. The invention discloses a novel and effective treatment way for treating or preventing cardiac trauma caused by chemotherapeutics.

Description

technical field [0001] The invention relates to a new application of prohematoxylin A derivatives, in particular to the application of prohematoxylin A derivatives in protecting heart damage caused by chemotherapeutic drugs. The invention belongs to the technical field of medicine. Background technique [0002] At present, the incidence of tumors is getting higher and higher, and people's quality of life is greatly reduced. However, with the continuous improvement of medical technology research and the wide application of anti-tumor drugs, the survival time of cancer patients has been significantly prolonged, which can be accompanied by Yes, some tumors can directly involve the cardiovascular vessels and their subsidiary structures, causing serious harm. In addition, the potential cardiovascular damage and cardiovascular system diseases caused by tumor treatment have become health problems that cannot be ignored for cancer patients. [0003] There are four commonly used che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P9/00A61P9/04
CPCA61K31/12
Inventor 张毛毛吴健于波
Owner HARBIN MEDICAL UNIVERSITY
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