Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of microbial fertilizer special for root swelling of rehmannia glutinosa

A biological bacterial fertilizer and special-purpose technology, which is applied in the field of preparation of special biological bacterial fertilizer for root expansion of Rehmannia glutinosa, can solve rare and other problems

Inactive Publication Date: 2017-12-22
HENAN NORMAL UNIV
View PDF15 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, microbial fertilizer products that are specially used to stimulate the expansion of the roots of Rehmannia glutinosa are still relatively rare in the market.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of microbial fertilizer special for root swelling of rehmannia glutinosa
  • Preparation method of microbial fertilizer special for root swelling of rehmannia glutinosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] (1) Preparation of seed solution: Bacillus licheniformis, Pichia pastoris and Trichoderma harzianum were isolated from the rhizosphere soil of Rehmannia glutinosa, and the Bacillus licheniformis strain was inoculated in LB liquid medium, at 37°C, 180rpm Cultivate under conditions for 48 hours, wherein the composition of LB liquid medium is calculated in g / L: sodium chloride 10, yeast powder 5, peptone 10, and the rest is water, pH 7.0, sterilized at 121°C for 20 minutes; Pichia pastoris Bacterial strains were inoculated in YPD liquid medium and cultured at 28°C and 180rpm for 48 hours. The composition of YPD liquid medium was calculated in g / L: glucose 20, peptone 10, yeast extract 5, and the rest was water. Natural pH, sterilized at 115°C for 30 minutes; Trichoderma harzianum was activated with PDA medium and bran medium was used. After 7 days of cultivation, the medium was rinsed with sterile water, filtered, and spores were obtained. The components of PDA medium were ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a microbial fertilizer special for root swelling of rehmannia glutinosa, belongs to the technical field of preparation of microbial fertilizers. A technical scheme of the invention is that the preparation method comprises the following steps: separating from rhizosphere soil of rehmannia glutinosa to obtain bacillus licheniformis strains, pichia pastoris strains and trichoderma harzianum; mixing three microbial cultures at a mass ratio of 1: 1: 1 to prepare seed liquid; adjusting a proportion of cow dung to straws according to a carbon-nitrogen ratio of the cow dung to the straws so that the carbon-nitrogen ratio after mixing is 20-25%; controlling a water content at 50%-60%; applying the prepared seed liquid to a mixture of the cow dung and the straws, wherein the inoculation amount is 1 wt%; mixing uniformly and then piling up to form strip-shaped stacks; covering with plastic films for fermentation; turning the stacks during fermentation; and fermenting for 35 days to pile the microbial fertilizer. The microbial fertilizer effectively stimulates the root swelling of rehmannia glutinosa, reduces use of fertilizers, and realizes resource reuse of the cow dung and the straws.

Description

technical field [0001] The invention belongs to the technical field of preparation of microbial bacterial fertilizer, and in particular relates to a preparation method of special biological bacterial fertilizer for root expansion of rehmannia glutinosa. Background technique [0002] Rehmannia glutinosa has a long history of cultivation in my country, and its root is a famous and precious traditional Chinese medicine with important economic value. Existing records show that Rehmannia glutinosa prepared by different methods has different application values ​​in clearing away heat, cooling blood, moistening dryness and nourishing yin. Modern research has extended the medicinal value of Rehmannia glutinosa to anti-inflammatory, anti-tumor, and prevention of insomnia. The quality of Rehmannia glutinosa is subject to strong regional restrictions. Jiaozuo City, Henan Province has been considered to have the best environmental and climatic conditions for growing Rehmannia glutinosa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C05F15/00C05F11/08C12N1/20C12N1/14C12N1/16C12R1/10C12R1/885C12R1/84
CPCC05F3/00C05F11/08C12N1/14C12N1/16C12N1/20C05F11/00C05F17/00
Inventor 杨清香王瑞飞康春晓王妍李明军
Owner HENAN NORMAL UNIV