Preparation method of plural gel and application thereof on induction in chondrogenic differentiation of stem cells

A composite gel and collagen technology, which is applied in the fields of medicine and biomedical engineering, can solve the problems of lack of blood supply to articular cartilage, inability to move damaged parts for repair, and difficulty in obtaining satisfactory clinical effects, so as to promote cell proliferation and differentiation, improve curative effect, The effect of prolonged release

Active Publication Date: 2017-12-29
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Articular cartilage defect has always been a difficult problem in orthopedic clinical treatment, mainly because articular cartilage has no blood supply, and chondrocytes are buried in a thick extr...

Method used

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  • Preparation method of plural gel and application thereof on induction in chondrogenic differentiation of stem cells
  • Preparation method of plural gel and application thereof on induction in chondrogenic differentiation of stem cells
  • Preparation method of plural gel and application thereof on induction in chondrogenic differentiation of stem cells

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Experimental program
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Effect test

Embodiment 1

[0040] The preparation of the composite gel comprises the following steps: (1) preparation of collagen, taking 15 mg of aseptic cowhide collagen freeze-dried product and adding 1 mL of acetic acid with a concentration of 0.5 M, after swelling, stirring continuously on ice to fully dissolve the collagen; 2) Preparation of genipin plain solution, weigh 2 mg of genipin and dissolve in 2 μL of absolute ethanol, add PBS to make up to 15 μL; (3) Preparation of composite gel, take 100 μL of collagen prepared by step (1), and then Add 1 μL each of the genipin stock solution prepared in step (2) and carbon dots, mix thoroughly, and then place it at a constant temperature of 37°C for 15 minutes to obtain genipin-collagen-carbon dots composite gel A.

Embodiment 2

[0042] Preparation of composite gel: including the following steps: (1) preparation of collagen, take 10 mg of aseptic cowhide collagen freeze-dried product and add 1 mL of acetic acid with a concentration of 0.4 M, after swelling, stir continuously on ice to fully dissolve the collagen; 2) Preparation of genipin plain solution, weigh 2 mg of genipin and dissolve in 2 μL of absolute ethanol, add PBS to make up to 15 μL; (3) Preparation of composite gel, take 120 μL of collagen prepared by step (1), and then Add 1.5 μL each of the genipin stock solution prepared in step (2) and carbon dots, mix well, and then place it at a constant temperature of 37°C for 15 minutes to obtain genipin-collagen-carbon dots composite gel B.

Embodiment 3

[0044] Preparation of composite gel: including the following steps: (1) preparation of collagen, take 20 mg of aseptic cowhide collagen freeze-dried product and add 1 mL of acetic acid with a concentration of 0.6 M, after swelling, stir continuously on ice to fully dissolve the collagen; 2) Preparation of genipin plain solution, weigh 2 mg of genipin and dissolve in 6 μL of absolute ethanol, add PBS to make up to 15 μL; (3) Preparation of composite gel, take 80 μL of collagen prepared by step (1), and then Add 0.5 μL each of the genipin stock solution prepared in step (2) and carbon dots, mix thoroughly, and then place it at a constant temperature of 37°C for 15 minutes to obtain genipin-collagen-carbon dots composite gel C.

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Abstract

The invention discloses a preparation method of plural gel and application thereof on induction in chondrogenic differentiation of stem cells. The method comprises the following steps: (1) preparation of collagen: preparing a sterile cowhide collagen lyophilized product-acetic acid solution with the concentration of 10-20 mg/mL by using acetic acid with the concentration of 0.4-0.6 M as a solvent; (2) preparation of a genipin stock solution; and (3) taking a certain volume of the collagen prepared in the step (1), adding a certain volume of the genipin stock solution prepared in the step (2) and carbon dots, and preparing a mixed solution of genipin and the carbon dots of which the concentrations are separately 0.4-2 vol%, and fully mixing uniformly, and then standing for 15 minutes at the constant temperature of 37 DEG C to obtain genipin-collagen-carbon dot plural gel. By genipin cross-linking, ROS releasing of the carbon dots is limited, ROS released by the carbon dots is controlled to be in a low amount state, the promotion effect of the carbon dots to cell proliferation and differentiation is played, and moreover, the time effect of action of the carbon dots is maintained. The plural gel can be used for inducing stem cells to be differentiated into chondroblasts.

Description

technical field [0001] The invention relates to the fields of medicine and biomedical engineering, in particular to a preparation method of genipin-collagen-carbon dot composite gel and its application in inducing stem cells to differentiate into cartilage. Background technique [0002] Articular cartilage defect has always been a difficult problem in orthopedic clinical treatment, mainly because articular cartilage has no blood supply, and chondrocytes are buried in a thick extracellular matrix, which cannot move to the damaged site to participate in repair. Traditional treatment methods such as intra-articular debridement and lavage are difficult to obtain satisfactory clinical results. [0003] Tissue engineering technology constructs tissue-engineered cartilage by seeding cells in conformity with scaffold materials and implanting it into the defect site to achieve therapeutic purposes. In recent years, with the rise of nanotechnology, its application in the field of reg...

Claims

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Application Information

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IPC IPC(8): C08J3/09C08J3/24C08L89/00C08K3/04A61L27/08A61L27/24A61L27/52A61L27/54
CPCA61L27/08A61L27/24A61L27/52A61L27/54A61L2300/108A61L2300/412A61L2430/06C08J3/09C08J3/24C08J2389/00C08K3/04C08L89/00
Inventor 郑立赵劲民陆真慧秦雄吴洋许富本
Owner GUANGXI MEDICAL UNIVERSITY
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