Preparation method and application of a tumor cell spheroid

A technology of tumor cells and drugs, applied in the field of biomedicine, can solve the problems of easy error and high price of Transwell chamber, and achieve the effect of low cost, fast speed and high efficiency
CN107523544BActive Publication Date: 2020-03-31THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
2 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Publication Date
2020-03-31

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the field of a biological medicine, and particularly relates to a preparation method of a cancer cell ball and an application. The preparation method is carried out according to the steps of firstly, dripping cancer cell suspension fluid in a cell culture plate one by one; then dripping PBS solution at the corresponding position of a plate cover; backing off the culture plate on the plate cover to cultivate to obtain a ball-shaped cancer cell; secondly, removing PBS, and placing the culture plate on an ice; adding Matrigel in holes one by one and culturing to form a cancer cell ball coated by the Matrigel. The cancer cell ball can be used for judging the migration and invasion abilities of the cancer cell and screening micromolecule drugs related to the migration and invasion of the cancer cell. The cancer cell ball is applied to the experiment of the migration and invasion of the cancer cell; the preparation method is simple in operation, quick in speed, high in efficiency, and low in cost; the cancer cell ball can be used at a lab or a company where the cell operation can be carried out.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method of a tumor cell sphere and its application in tumor cell migration and invasion experiments. Background technique

[0002] Tumor invasion and metastasis (invasion&metastasis) are important features of many tumors in the middle and late stages, and are also the main cause of clinical death for most patients. The essence of tumor invasion and metastasis is actually the migration and invasion of tumor cells. At present, a large number of scientific studies on the ability to inhibit tumor cell migration and invasion have been carried out at home and abroad, and many genes related to migration and invasion have been discovered, such as KAI-1, nm23, KISS-1 and BrMS1, etc. potential target.

[0003] In many anti-tumor preparations, such as recombinant cytokines / antibodies, adoptive cell therapy and therapeutic vaccines, and small molecule drugs, small molecu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More