Biological information processing method for analyzing circulating tumor DNA (deoxyribonucleic acid)

An information processing method and biological technology, applied in the field of biological information processing of circulating tumor DNA analysis, can solve problems such as improper treatment and overtreatment, and achieve the effects of increasing reliability, reducing false positives, and simple and convenient sample collection

Inactive Publication Date: 2017-12-29
HANGZHOU HEYI GENE TECH
View PDF13 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the heterogeneity of tumors and individual differences among populations, tumor samples of the same tissue in different populations, different genders, and even different individuals in different living environments will have different genetic backgrounds. Therefore, it is particularly necessary to obtain individual genetic information

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological information processing method for analyzing circulating tumor DNA (deoxyribonucleic acid)
  • Biological information processing method for analyzing circulating tumor DNA (deoxyribonucleic acid)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] Embodiments of the present invention are described in further detail below in conjunction with accompanying drawings:

[0042] The present invention is a biological information processing method for circulating tumor DNA analysis, comprising the following steps:

[0043] (1) cfDNA extraction, library construction and sequencing:

[0044] Using the magnetic bead method to extract cfDNA in the sample plasma for sample library construction; the sample plasma is derived from human plasma;

[0045] Sequencing adapters are added to both ends of 100-300bp cfDNA molecules. The sequencing adapters contain 8bp known tag sequences and 8bp random tag sequences. Molecular probes are used to hybridize and capture target DNA fragments to complete library construction;

[0046] Use a next-generation sequencer to perform double-end sequencing on the constructed library, and the sequencing read length is 150bp; the next-generation sequencer is an illuminaNextSeq CN500 sequencer, BGISEQ-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a biological information processing method for analyzing circulating tumor DNA (deoxyribonucleic acid). The method includes 1), performing cfDNA extraction, database creating and sequencing; 2), performing sequencing-data quality control and sequencing comparison; 3), performing sequencing data correction; 4), using two pieces of software to simultaneously perform gene mutation detection on the sequencing data corrected in the step 3) and summing and integrating analysis results of the software; 5), establishing a mutant trusted data set by the aid of a sequence corrected in the step 3) and using the data set to provide credibility support to a mutation result acquired in the step 4). The ctDNA in the cfDNA is taken as a detection object, detection can be performed by only collecting a small amount of peripheral blood of a subject, and conciseness and convenience in sample collection are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, more specifically, it relates to a biological information processing method for circulating tumor DNA analysis. Background technique [0002] In recent years, due to environmental pollution and bad living habits, the morbidity and mortality of tumors have continued to increase, which has become the number one factor that endangers human health and survival. It is estimated that in the next 20 years, the number of new cancer cases in the world will reach 22 million each year, and the number of cancer deaths will also soar to 13 million during the same period. On average, 1 out of every 8 deaths will die from cancer. In China, 264 people per 100,000 people suffer from cancer every year, and 192 people per 100,000 people die of cancer every year. The 5-year survival rate of cancer patients in China is about 30%, far behind the 60% in developed countries, which shows that the situation of cancer prevent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/68C40B50/06G06F19/20
CPCC12N15/1013C12Q1/6869C12Q1/6886C12Q2600/156C40B50/06G16B25/00C12Q2535/122
Inventor 王军一叶克勇蔡庆乐
Owner HANGZHOU HEYI GENE TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap