The invention relates to a method and apparatus for measuring the thickness of the intermediate layer of a three-layer composite plate. By means of the interface wave transmitted between the interfaces, the intermediate layer of the three-layer composite plate is excited to generate and receive a guided wave signal, and by means of the guided wave signal, the interface wave velocity is measured, and by changing the frequency of the excitation signal several times, the dispersion curve of the interface wave transmitting in the composite plate can be obtained, and the group velocity stable corner frequency can be found; By means of the consistence of the interface wave group velocity and the phase velocity stable corner frequency, by taking the product (namely frequency thickness product) of the phase velocity stable corner frequency and the thickness of the intermediate layer of the three-layer composite plate as a constant value, and by means of the principle that the phase velocity stable corner frequency is related to the thickness of the intermediate layer, in the case of known stable corner frequency, the frequency thickness product is divided by the stable corner frequency to obtain the accurate thickness of the intermediate layer of the three-layer composite plate The measurement method is simple and easy, high in measurement precision, and reliable in measurement result, and is suitable for the accurate measurement of the intermediate layer of the three-layer composite plate.