Biological tissue sequence slice microscopic image registration method and apparatus

Abstract

The invention relates to the field of image processing and neurosciences, in particular to a biological tissue sequence slice microscopic image registration method and apparatus, and aims to solve theproblem that real and reliable corresponding point information cannot be found between adjacent biological tissue slice microscopic images. For the purpose, the invention provides the biological tissue sequence slice microscopic image registration method. The method comprises the steps of obtaining corresponding points of adjacent microscopic images in biological tissue sequence slice microscopicimages; adjusting the positions of the obtained corresponding points; and according to the adjusted positions of the corresponding points, performing image deformation on the biological tissue sequence slice microscopic images. Through the method and the apparatus, the real and reliable corresponding point information between the adjacent slice microscopic images can be found, so that the precision of biological tissue sequence slice microscopic image registration is ensured.

Description

technical field [0001] The invention relates to the fields of image processing and neuroscience, in particular to a method and device for registering microscopic images of serial slices of biological tissues. Background technique [0002] At present, the three-dimensional reconstruction methods of microscopic images of biological tissue serial slices based on electron microscope imaging technology mainly include sequential cross-sectional imaging methods and serial slice imaging methods. Among them, the steps of the sequential cross-sectional imaging method mainly include: fixing the biological tissue sample in the sample chamber of the electron microscope, removing the sample surface with a thickness of ten nanometers by cutting with a diamond knife or bombarding with an ion beam after imaging the surface, and again processing The samples that have been processed are imaged, and the above process is repeated until images of all samples are obtained. The image sequence obta...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned problems in the prior art, that is, to solve the technical problem that it is impossible to find true and reliable corresponding point information between adjacent slice microscopic images, one aspect of the present invention proposes a biological tissue serial slice microscopic Image registration methods, including:

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