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Sterile rapid sprouting method for hylocereus undatus seeds

A technology for dragon fruit and seeds, which is applied in the fields of seed and rhizome treatment, botanical equipment and methods, and chemicals for biological control, etc. Effects of seed germination, improvement of germination rate, seedling growth and induction of adventitious buds

Inactive Publication Date: 2018-01-09
GUANGXI SHATIAN XIANRENTAN AGRI INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficient cultivation of dragon fruit seedlings is the basis of hybrid breeding, but there are problems such as slow seed germination, low germination rate, irregular germination, and thin seedlings in direct sowing seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of aseptic quick germination method of dragon fruit seed, comprises the following steps:

[0021] (1) Pitaya seeds pretreatment: Rinse the dragon fruit seeds under running water, and then wash them with 15% NaClO 3 Soak and sterilize for 15 minutes, rinse with sterile water for 5 times, then soak with seed soaking agent for 12 hours, take out and dry the surface water with sterile filter paper;

[0022] (2) Seed germination: First, sterilize the MS medium at 125°C for 20 minutes, and adjust the pH value of the MS medium to 5.8, peel off the seed coat of the pitaya seeds treated in step (1) and inoculate them after sterilization Then put them into a constant temperature incubator for cultivation. When inoculating, the seeds are placed 2-3cm below the medium, and the culture temperature is controlled at 25±2°C, and the light intensity is 33μmol / ( m 2 s), with 14 hours of light per day, and the germination can be stopped after the seeds are white;

[0023] Wherein...

Embodiment 2

[0027] A kind of aseptic quick germination method of dragon fruit seed, comprises the following steps:

[0028] (1) Pitaya seeds pretreatment: Rinse the dragon fruit seeds under running water, and then wash them with 15% NaClO 3 Soak and sterilize for 12 minutes, rinse with sterile water for 4 times, then soak with seed soaking agent for 24 hours, take out and dry the surface water with sterile filter paper;

[0029] (2) Seed germination: First, sterilize the MS medium at 126°C for 20 minutes, and adjust the pH value of the MS medium to 5.8. Peel off the seed coat of the pitaya seeds treated in step (1) and inoculate them after sterilization Then put them into a constant temperature incubator for cultivation. When inoculating, the seeds are placed 2-3cm below the medium, and the culture temperature is controlled at 25±2°C, and the light intensity is 30μmol / ( m 2 s), with 18 hours of light per day, and the germination can be stopped after the seeds are white;

[0030] Wherein...

Embodiment 3

[0034] A kind of aseptic quick germination method of dragon fruit seed, comprises the following steps:

[0035] (1) Pitaya seeds pretreatment: Rinse the dragon fruit seeds under running water, and then wash them with 15% NaClO 3 Soak and sterilize for 12 minutes, rinse with sterile water for 4 times, then soak with seed soaking agent for 10 hours, take out and dry the surface water with sterile filter paper;

[0036] (2) Seed germination: First, sterilize the MS medium at 126°C for 30 minutes, and adjust the pH value of the MS medium to 5.8, peel off the seed coat of the pitaya seeds treated in step (1) and inoculate them on the medium Then put it into a constant temperature incubator and cultivate it. When inoculating, the seeds are placed 2-3cm under the culture medium, and the culture temperature is controlled to be 25±2°C, and the light intensity is 30-35μmol / (m 2 s), with 12 hours of light per day, and the germination can be stopped after the seeds are white;

[0037] W...

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PUM

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Abstract

The invention discloses a sterile rapid sprouting method for hylocereus undatus seeds. The method comprises the following steps: (1) pretreating the hylocereus undatus seeds, namely flushing and cleaning the hylocereus undatus seeds with running water, soaking in 15% NaClO3 and sterilizing for 12-15min, flushing for 4-5 times by using sterile water, soaking for 10-24h by using a seed soaking agent, taking out and absorbing the surface water by using sterile filtering paper; (2) sprouting the seeds, namely peeling the hylocereus undatus seeds treated in the step (1), inoculating onto a culturemedium and then putting into a constant-temperature incubation box for culturing, placing the seeds to the position 2-3cm lower than the culture medium while inoculating, controlling the culturing temperature to be 25+ / -2 DEG C and illumination intensity to be 30-35 mumol / (m<2>.s), daily illuminating for 12h-18h and stopping sprouting after the seeds show white color. According to the sterile rapid sprouting method for hylocereus undatus seeds disclosed by the invention, the seedling plants can be quickly and efficiently acquired, meanwhile the germination ratio can be greatly increased, the culturing time of the seedling plants can be shortened and the problems of slow seed germination, low germination ratio, irregular germination, weak seedlings, and the like can be solved.

Description

technical field [0001] The invention belongs to the technical field of plant seed germination, and in particular relates to a method for rapid aseptic germination of dragon fruit seeds. Background technique [0002] Pitaya belongs to Hylocereus undatus (Cactaceae) or Seleniereus mejalantous (Seleniereus mejalantous) plant. Its fruit is a health food with high vitamins, low sugar and low fat. It is a new tropical, Subtropical fruit tree with high economic value. The efficient cultivation of dragon fruit seedlings is the basis of hybrid breeding, but direct sowing seedlings have problems such as slow seed germination, low germination rate, irregular germination, and thin seedlings. Contents of the invention [0003] In order to solve the above-mentioned deficiencies in the prior art, the invention provides a kind of aseptic rapid germination method of pitaya seeds, which can quickly and efficiently obtain seed plants, greatly improve the germination rate, and shorten the cu...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01N43/12A01N45/00A01N37/12A01N43/90A01P21/00C05G3/00
Inventor 黎宇毅李灵洁陆世吉
Owner GUANGXI SHATIAN XIANRENTAN AGRI INVESTMENT CO LTD
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