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Application of water extract of flowers of Apios americana Medikus to mitigation of lipidosis in hepatocytes

A technology of lipid deposition and water extract, which is applied in the field of medicine, can solve problems such as easy rebound and lower immunity of the body, and achieve the effects of expanding application fields, reducing lipid deposition in LO2 cells, and good development prospects

Active Publication Date: 2018-01-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the other weight loss and fat reduction methods also have the disadvantages of lowering the body's immunity and easy rebound.

Method used

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  • Application of water extract of flowers of Apios americana Medikus to mitigation of lipidosis in hepatocytes
  • Application of water extract of flowers of Apios americana Medikus to mitigation of lipidosis in hepatocytes
  • Application of water extract of flowers of Apios americana Medikus to mitigation of lipidosis in hepatocytes

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1, a kind of preparation method of taro flower water extract, carries out following steps successively:

[0058] 1) Mix taro flowers and water at a ratio of 1g:10ml, and use a juice extractor to beat; the obtained slurry is leached by ultrasonic (40,000Hz ultrasonic frequency) for 90min at 45°C, and the extract is centrifuged at 3000g for 30min ,

[0059] The filter residue obtained by centrifugation replaces the taro flower in the above steps and repeats the beating, ultrasonic extraction and centrifugation of the above steps; the number of repetitions is 2 times;

[0060] The supernatants obtained by the above 3 times of centrifugation were combined to carry out the following step 2).

[0061] 2), the obtained supernatant was concentrated by rotary evaporation in a rotary evaporator (the process parameters set by the rotary evaporation were temperature 38°C, vacuum degree -0.09MPa) until it was 15% of the original volume;

[0062] 3), the concentrate obta...

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Abstract

The invention discloses application of the water extract of flowers of Apios americana Medikus to mitigation of lipidosis in hepatocytes. A preparation method for the water extract of flowers of Apiosamericana Medikus comprises the following steps: 1) mixing the flowers of Apios americana Medikus with water and successively carrying out smashing, ultrasonic extraction and centrifugation to obtaina supernatant; 2) subjecting the supernatant to rotary evaporation and concentration until the volume of the supernatant is reduced by 80 to 90%; 3) subjecting a concentrate obtained in the step 2) to separation and purification via macroporous resin with an aqueous formic acid solution with a volume concentration of 1% as a rinsing agent and an aqueous methanol solution containing 0.1% of formicacid as an eluting agent, and collecting eluate; 4) subjecting the eluate to rotary evaporation and concentration until the volume of the eluate is reduced by 80 to 90% so as to obtain slurry-like concentrate; and 5) pre-freezing the slurry-like concentrate, and then drying the pre-frozen slurry-like concentrate into powder with a vacuum lyophilizer so as to obtain the water extract of the flowers of Apios americana Medikus.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of the water extract of beetroot flower in reducing lipid deposition in liver cells. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitamin E and minerals. Among th...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P1/16A23L33/105A61K133/00
Inventor 郑晓冬楚强杨芸芸
Owner ZHEJIANG UNIV
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