Method for performing CASP3 gene knockout on mesenchymal stem cell through CRISPR-CAS system

A cell gene and stem cell technology, applied in the field of CASP3 gene editing, can solve the problem that siRNA cannot stabilize inheritance, and achieve the effect of high knockout efficiency and stable passage
CN107586779AActive Publication Date: 2018-01-16天津金匙医学科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
天津金匙医学科技有限公司
Publication Date
2018-01-16

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Abstract

The invention provides CASP3 gene editing performed on a mesenchymal stem cell through a zsystem, and particularly relates to the establishment of a mesenchymal stem cell line for constructing CASP3 gene knockout. Novel enhancing CREnhancer1.0 is adopted, and CRISPR-cas9 gene editing efficiency in a cell can be obviously improved. Bone mesenchymal stem cell CASP3 gene knocked out plasmid has goodhereditary stability.
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Description

technical field

[0001] The present invention provides a CRISPR-cas system for performing CASP3 gene editing on mesenchymal stem cells, and in particular relates to the establishment of a CASP3 gene knockout mesenchymal stem cell line. Background technique

[0002] Mesenchymal stem cells (MSCs) are adult stem cells with self-replication ability and multi-directional differentiation potential, which can develop into bone, cartilage, fat and other types of cells. Mesenchymal stem cells can be transplanted, and the type of cells they grow into depends on where they are injected. For example, mesenchymal stem cells injected into the heart can form healthy new tissue, among other things.

[0003] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells derived from the mesoderm and ectoderm in early development. Mainly exist in connective tissue and interstitium of organs, and the content is most abundant in bone marrow tissue. Since bone marrow is its main source, the...

Claims

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