Primers for real-time fluorescent quantitative PCR (polymerase chain reaction) assay of novel pigeon adenovirus EvaGreen

Abstract

The invention provides primers for the real-time fluorescent quantitative PCR (polymerase chain reaction) assay of novel pigeon adenovirus EvaGreen, the sequences of which are shown as SEQ ID NO.1-2 and which have high specificity and sensitivity. An established method can assay the infection of the novel pigeon adenovirus in a pigeon flock, the operation procedure is simplified, and the cost is reduced. After real-time fluorescent quantitative PCR is complete, by observing solubility curve peaks (Tm value), a result can be directly judged. The establishment of the invention can fill the gap of the related field at home and abroad.

Description

technical field [0001] The invention provides primers for real-time fluorescent quantitative PCR detection of new pigeon adenovirus EvaGreen, which belongs to the field of animal infectious diseases. Background technique [0002] Real-time fluorescence quantitative PCR method (Real time PCR) is a method of detecting the total amount of products after each cycle of polymerase chain reaction (PCR) with fluorescent chemical substances in DNA amplification reaction. A method for quantitative analysis of a specific DNA sequence in a sample to be tested by using an internal or external reference method. Real-time fluorescent quantitative PCR is to detect the progress of PCR in real time through fluorescent signals during the PCR amplification process. Due to the exponential phase of PCR amplification, there is a linear relationship between the Ct value of a template and the initial copy number of the template. Currently, in the non-specific fluorescent labeling of real-time fluo...

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Problems solved by technology

However, there are currently no primers research reports on the real-time fluorescent quantitative PCR detection method based on the saturated fluorescent dye Eva Green to detect pigeon new adenoviruses at home and abroad. The establishment of the present invention can fill in the research gaps in related fields at home and abroad

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