Microfluidics device for cell sorting and preparation method thereof
A microfluidic device and cell technology, applied in the field of cell sorting, can solve the problems of complex structure and high equipment cost of cell sorting devices, and achieve the effect of simple structure and low cost
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[0060] As a preferred embodiment of the present invention, the preparation method of the mold includes the following steps: coating a photoresist on the surface of the mold base, and obtaining a cavity structure corresponding to the lower base structure after exposure and development; optionally, coating The thickness of the overlying photoresist is 8-14 microns. In a preferred embodiment of the present invention, the mold is obtained by coating photoresist on a silicon wafer, exposing and developing, and the method is simple in operation and easy to realize.
[0061] In the above preferred embodiments, the thickness of the coated photoresist is typically but not limited to, for example: 8 microns, 10 microns, 11 microns, 12 microns, 13 microns or 14 microns.
[0062] As a preferred embodiment of the present invention, after the lower substrate is fabricated, use a puncher to punch holes in the lower substrate to prepare cell sample inlets, medium injection ports and sample ou...
Embodiment 1
[0065] Such as figure 1 As shown, the present embodiment is a microfluidic device for cell sorting, including a lower base 10 and an upper base connected to the lower base 10, the upper base is a glass slide, and the lower base 10 is provided with a cell for sorting. The microcolumn array 11 of the cell, along the cell flow direction, the angle between the arrangement direction of the columns 12 in the microcolumn array 11 and the cell flow direction is 2.8°; along the direction perpendicular to the cell flow direction, the angle between the columns 12 in the microcolumn array 11 The pitch is 10 microns. The micropillar array 11 in this embodiment is a group, and the pillars 12 in the micropillar array 11 are circular pillars. Along the cell flow direction, one end of the lower base body 10 is provided with a cell sample inlet 13 and a medium injection port 14, the cell sample inlet 13 is used to connect the cell tank, and the medium injection port 14 is used to connect the m...
Embodiment 2
[0068] This embodiment is a microfluidic device for cell sorting. The difference from Embodiment 1 is that along the direction of cell flow, the angle between the arrangement direction of the columns in the microcolumn array and the direction of cell flow is 9°; In the direction of cell flow, the distance between the pillars in the micropillar array is 12 micrometers.
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