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Induction culture medium and induction method for differentiating umbilical stalk mesenchymal stem cells into neuron-like cells

A technology for inducing culture medium and stromal cells, which is applied in the field of induction medium for umbilical cord mesenchymal stem cells to differentiate into neural-like cells, which can solve the problems of limited sources and achieve the effect of short induction time.

Active Publication Date: 2018-01-26
广州市天河诺亚生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of treating nervous system diseases is mostly autologous nerve transplantation, but the source of autologous nerve transplantation is limited, so finding cells from other sources is the key to cell transplantation and treatment of nervous system diseases
At present, there is no report that picatanol can make mesenchymal stem cells differentiate into neural-like cells

Method used

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  • Induction culture medium and induction method for differentiating umbilical stalk mesenchymal stem cells into neuron-like cells
  • Induction culture medium and induction method for differentiating umbilical stalk mesenchymal stem cells into neuron-like cells
  • Induction culture medium and induction method for differentiating umbilical stalk mesenchymal stem cells into neuron-like cells

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Embodiment 1

[0031] The present invention is an example of an induction medium for umbilical cord mesenchymal stem cells to differentiate into nerve-like cells, and the induction medium for umbilical cord mesenchymal stem cells to differentiate into nerve-like cells in this example includes piceatanol and basic medium , the concentration of piceatanol in the induction medium is 62.5 μmol / L, and the basic medium is L-DMEM medium.

[0032] The method for inducing differentiation of umbilical cord mesenchymal stem cells into neural-like cells in this example is as follows:

[0033] ① Isolation, culture and expansion of umbilical cord mesenchymal stem cells

[0034] Under sterile conditions, take a 10cm umbilical cord from a healthy fetus, soak it in a petri dish filled with alcohol for one minute, then put it into a petri dish filled with normal saline to wash away blood clots and residual alcohol; then put it into the next petri dish filled with In a petri dish of normal saline, cut the w...

Embodiment 2

[0049] The present invention is an example of an induction medium for umbilical cord mesenchymal stem cells to differentiate into nerve-like cells, and the induction medium for umbilical cord mesenchymal stem cells to differentiate into nerve-like cells in this example includes piceatanol and basic medium , the concentration of piceatanol in the induction medium is 125 μmol / L, and the basic medium is L-DMEM medium.

[0050] The specific operation of the method for inducing differentiation of umbilical cord mesenchymal stem cells into neural-like cells in this example is the same as in Example 1, except that the concentration of piceatanol in the induction medium used is 125 μmol / L.

[0051] Results: Adding L-DMEM medium containing 125 μmol / L picatatanol for induction, 90% of neural-like cells could be obtained after only 2 hours of induction of mesenchymal stem cells.

[0052] When induced by 125μmol / L piceatanol for 0.5h, about 45% of the cell body shrank into a round or oval...

Embodiment 3

[0054] The present invention is an example of an induction medium for umbilical cord mesenchymal stem cells to differentiate into nerve-like cells, and the induction medium for umbilical cord mesenchymal stem cells to differentiate into nerve-like cells in this example includes piceatanol and basic medium , the concentration of piceatanol in the induction medium is 250 μmol / L, and the basic medium is L-DMEM medium.

[0055] The specific operation of the method for inducing the differentiation of umbilical cord mesenchymal stem cells into neural-like cells in this example is the same as in Example 1, except that the concentration of piceatanol in the induction medium used is 250 μmol / L.

[0056] Results: Adding L-DMEM medium containing 250 μmol / L picatatanol for induction, 90% of neural-like cells can be obtained after only 2 hours of induction of mesenchymal stem cells.

[0057] After being induced by 250μmol / L piceatanol for 0.5h, the cell body of about 60% of the cells shran...

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Abstract

The invention belongs to the field of biotechnology, and specifically relates to an induction culture medium and an induction method for differentiating umbilical stalk mesenchymal stem cells into neuron-like cells. The invention provides the induction culture medium and induction method for differentiating umbilical stalk mesenchymal stem cells into neuron-like cells. The induction culture mediumcomprises piceatannol and a base culture medium, and the concentration of piceatannol in the induction culture medium is 62.5-250mu mol / L. According to the induction method, the umbilical stalk mesenchymal stem cells are cultivated in the induction culture medium and are differentiated directionally so as to obtain neuron-like cells. The induction method costs a short induction time, and can generate neuron-like cells with neurons and neuroglia through induction, thereby providing a new cell source for damage repairing of a central nervous system.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an induction medium and an induction method for differentiating umbilical cord mesenchymal stem cells into nerve-like cells. Background technique [0002] Nervous system diseases seriously threaten human health, such as the well-known Parkinson's disease and Alzheimer's disease, as well as hemorrhagic stroke, traumatic diseases and spinal cord injuries, all of which are due to the failure of differentiated and mature nerve cells to divide and proliferate to compensate caused by damaged and dead cells. At present, autologous nerve transplantation is mostly used to treat nervous system diseases, but the source of autologous nerve transplantation is limited, so finding cells from other sources is the key to cell transplantation and treatment of nervous system diseases. [0003] Mesenchymal stem cells (mesenchymal stem cells, MSCs) are pluripotent stem cells with high self-r...

Claims

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Application Information

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IPC IPC(8): C12N5/079C12N5/0793
Inventor 魏伟嵐山芮王雅茹
Owner 广州市天河诺亚生物工程有限公司
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