A kind of extraction preparation method of zeatin in biogas slurry concentrate
A technology of concentrated liquid and zeatin, applied in the direction of organic chemistry, can solve the problems of reducing the accuracy of biogas slurry composition research, complex biogas slurry composition, etc., achieving considerable economic and practical value, wide application prospects, and high yield.
Active Publication Date: 2019-11-29
SHANDONG MINHE BIOTECHNOLOGY CO LTD
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
At present, the existing research on biogas slurry is mainly to do simple treatment of biogas slurry (such as glass fiber, etc.) and then directly measure it with high-efficiency liquid phase. influence, which will reduce the accuracy of biogas slurry composition research
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0034] (1) Sample processing
[0035] Take 200g of biogas slurry, dry it in an oven and weigh it. Weigh an appropriate amount of dry matter, place it in a round bottom flask, add 200ml of methanol, heat to reflux for 2h, twice. After concentrating and drying the extract solution, extract it with an appropriate amount of ethyl acetate for 3 times, and continue to extract the filter residue after ethyl acetate extraction for 3 times with an appropriate amount of n-butanol to obtain an n-butanol extract.
[0036] (2) Preparation of zeatin in biogas slurry
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to the field of separation preparation of concentrated solution components of biogas fluid, and particularly discloses an extraction preparation method of zeatin in a concentrated solution of biogas fluid. The method specially comprises the following steps: lixiviating solid substances obtained by drying the concentrated solution of the biogas fluid by using methanol to obtain a leaching solution; extracting a dried product of the leaching solution by using ethyl acetate to obtain filter residues; extracting the filter residues for several times by using normal butyl alcohol to obtain a normal butyl alcohol extracting solution; sampling the extracting solution on a silica gel column by using dichloromethane; by using ethyl acetate as an eluent, performing gradient elution according to volume ratios of 10 to 1, 8 to 1, 6 to 1, 4 to 1 and 2 to 1; performing collection by setting an isovolume as a fraction; determining and combining fractions with same spots, and performing separation and purification to obtain the zeatin. A purification method of the zeatin in the concentrated solution of the biogas fluid disclosed by the invention is easy to operate, so that the operation cost is low; therefore, the method has appreciable economical and practical values and wide application prospects.
Description
technical field [0001] The invention relates to the field of separation and preparation of components of biogas slurry concentrate, in particular to a method for extracting and preparing zeatin in biogas slurry concentrate. Background technique [0002] At present, there are few reports on the organic components of biogas slurry and biogas residue, and the compounds isolated from biogas slurry are mainly plant hormones, free amino acids, Vitamin B, antibiotics, 2-quinolinone and 17β-estradiol, They exhibit activities such as promoting crop growth and development, and antimicrobial activity. At present, the existing research on biogas slurry is mainly to do simple treatment of biogas slurry (such as glass fiber, etc.) and then directly measure it with high-efficiency liquid phase. It will reduce the accuracy of biogas slurry composition research. The method is used to treat the biogas slurry, removes a large amount of water, volatile amines and solid impurities in the bioga...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C07D473/34
CPCC07D473/34
Inventor 孙宪法张东明董泰丽
Owner SHANDONG MINHE BIOTECHNOLOGY CO LTD