biosynthetic cornea
A biosynthesis, corneal technology, used in tissue regeneration, medical science, prosthesis, etc., can solve the problem of corneal implant tensile strength reducing optical clarity and other problems
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example 1
[0048] Example 1. Biosynthetic cornea and production of ophthalmic devices.
[0049] The biosynthetic cornea of the present invention is composed of small diameter collagen microfibrils arranged in a highly dense ordered array, especially in a highly dense parallel array. The biosynthetic cornea of the present invention is an advancement in providing a higher degree of optical clarity compared to prior art synthetic replacement corneas. Surprisingly, the biosynthetic corneas of the present invention provide such superior optical clarity while providing higher collagen content and improved suturability compared to prior art synthetic replacement corneas. A comparison of the biosynthetic cornea of the present invention with synthetic replacement corneal inlays or implants (so-called "ophthalmic devices") disclosed in the art is provided to demonstrate these improved properties.
[0050] The biosynthetic corneas of the invention were prepared using the methods described he...
example 2
[0061] Example 2. Comparison between biosynthetic corneas and ophthalmic devices.
[0062] Transparency and clarity of biosynthetic corneas and ophthalmic devices are checked by visual inspection and light absorbance across the visible spectrum. like figure 1 As shown, sample A2 is more transparent and exhibits higher clarity than sample B2 by visual inspection.
[0063] For absorbance testing, samples A1, A2, B1 and B2 were equilibrated with PBS buffer at room temperature. The balanced sample was then placed in a 1.5ml semi-micro disposable cuvette (Brand GMBH&Co.KG, Germany), and scanned from 300nm to 800nm. Absorbance (optical density) is plotted against wavelength and used as an indicator of sample clarity. In general, the lower the absorbance of the sample, the greater the optical clarity. like figure 2 As shown in A, sample A1 has a lower absorbance profile than sample B1, indicating that the biosynthetic cornea of the present invention has improved optical clar...
example 3
[0065] Example 3. Crosslinking parameters for production of biosynthetic corneas.
[0066] In developing the biosynthetic corneas of the present invention, experiments were performed to identify the parameters required to reproducibly produce biosynthetic corneas with optical clarity, including high collagen content biosynthetic corneas with optical clarity. One particular parameter investigated was the pH sensitivity of the procedure used to create the biosynthetic cornea. The '490 publication generally states that "the pH used in making such devices is generally less than about 6.0, for example, the pH may be between about 5.0 and about 5.5" (see, e.g., page 19 of the '490 publication, lines 3 to 5 Row). Most of the examples provided in the '490 publication were performed at pH 5.5, while some examples simply stated that the pH was adjusted to about 5. No preference is disclosed.
[0067] To study the effect of the pH of the cross-linking reaction on the optical clarity o...
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