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A kind of rapid freeze-drying method of 50 parts of swine mycoplasma pneumonia live vaccine

A technology for mycoplasma swine pneumonia and live vaccines, which is applied in the direction of vaccines, veterinary vaccines, freeze-drying and transportation, etc. It can solve the problems of frequent dilution operations and achieve the effects of short running time and energy saving of equipment

Active Publication Date: 2021-02-09
兆丰华生物科技(南京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for quick freeze-drying of 50 parts of products of live vaccine of Mycoplasma swine pneumonia, so as to solve the problem of frequent dilution operation in the immunization process of 10 parts of products in the prior art

Method used

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  • A kind of rapid freeze-drying method of 50 parts of swine mycoplasma pneumonia live vaccine
  • A kind of rapid freeze-drying method of 50 parts of swine mycoplasma pneumonia live vaccine
  • A kind of rapid freeze-drying method of 50 parts of swine mycoplasma pneumonia live vaccine

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Experimental program
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Effect test

Embodiment 1

[0028] A kind of product fast freeze-drying method of 50 parts of swine mycoplasma pneumonia live vaccine comprises the following steps:

[0029] (1) Freeze-dried strains of the live vaccine of Mycoplasma swine pneumonia were resuscitated and subcultured, and the count of live bacteria selected for the antigen was ≥10 9 The CCU freeze-dries the product;

[0030] (2) Prepare gelatin sucrose freeze-drying protection agent, gelatin sucrose freeze-drying protection agent is prepared according to the content of 4% gelatin and 20% sucrose, after completely dissolving, it is subjected to 115 DEG C, 40 minutes autoclaving treatment;

[0031] (3) According to the volume ratio of antigen: protective agent is 3:1, mix thoroughly and evenly;

[0032] (4) Quantitatively pack the fully mixed product in 4ml / bottle, and put it on the freeze-drying box plate;

[0033] (5) Run the freeze-drying program:

[0034] ① Freezing: Firstly, under the condition of refrigerating the plate layer at 5°C...

Embodiment 2

[0040] Adopt the method of embodiment 1 to carry out rapid freeze-drying test to Nanjing Tianbang swine mycoplasma pneumonia live vaccine (168 strains)

[0041] Specific steps are as follows:

[0042] (1) The product antigen is resuscitated and subcultured with Nanjing Tianbang Live Mycoplasma Pneumonia Vaccine (168 strains) freeze-dried strains, and the count of viable bacteria in the antigen is ≥10 9 The CCU performs lyophilization of the product.

[0043] (2) Preparation of the freeze-drying protective agent: it is prepared according to the mass percentage content of 4% gelatin and 20% sucrose, and after being completely dissolved, it is subjected to high-pressure sterilization treatment at 115° C. for 40 minutes.

[0044] (3) Product ratio: Mix the antigen and protective agent thoroughly and evenly at a volume ratio of 3:1.

[0045] (4) Packing and boxing: Pack the well-mixed product quantitatively in 4ml / bottle, and place it in the freeze-drying box in time.

[0046] (...

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Abstract

The invention relates to the technical field of freeze-drying vaccines, in particular to a quick freeze-drying method for a live vaccine of mycoplasma pneumoniae in swine, comprising (1) resuscitation and subculture of the freeze-dried bacterial strain of the live vaccine of mycoplasma pneumoniae in swine, and selection of freeze-dried antigenic materials for the product; (2) Prepare gelatin sucrose freeze-drying protective agent; (3) fully mix the antigen and protective agent; (4) quantify the well-mixed product and place it on the plate of the freeze-drying box; (5) run the freeze-drying program ① Freezing: First, under the condition of cooling the plate layer at 5°C, cool down to -40°C within 1 hour and keep it for 4 hours; ②Vacuum: vacuumize the freeze-drying box to make it below 0.1mbar; ③Sublimation drying: vacuum Raise the temperature to 10°C within 1 hour under the condition of 0.07mbar, and keep it for 15 hours, and then keep it at 15°C for 1 hour; ④Secondary drying: raise the temperature to 32°C in 20 minutes, keep it for 5 hours, and continue for 20 minutes Internally raise the temperature to 36°C and keep it for 2 hours; ⑤Start the corking function, and press the freeze-dried seedlings and take them out of the box.

Description

technical field [0001] The invention relates to the technical field of vaccine freeze-drying, in particular to a quick freeze-drying method for a live vaccine of mycoplasma pneumoniae in swine. Background technique [0002] In recent years, the application of live vaccine against Mycoplasma suis pneumonia has gradually been paid attention to and widely used. The traditional production of 10-head products has frequent dilution steps in the process of immunization, which is obviously not conducive to the immunization operation of large-scale vaccination of whole litters and groups. [0003] In order to meet the needs of market-oriented development, the 50-head product is more suitable for use, saves manpower, and ensures the consistency of group vaccination. However, in order to fully ensure the effective antigen content of the product in the production process of small-capacity and high-dose products, it is necessary to establish an effective product freeze-drying method to e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K47/42A61K47/26A61K39/02A61P11/00A61P31/04
CPCA61K9/19A61K39/0241A61K47/26A61K47/42A61K2039/522A61K2039/552
Inventor 陈兆霞朱月婷徐霞李卫东朱晓玮
Owner 兆丰华生物科技(南京)有限公司
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