A preparation method of ciprofloxacin ratiometric fluorescent probe based on nanocomposite
A ratiometric fluorescent probe, nanocomposite technology, applied in nanotechnology, nanotechnology, nanotechnology and other directions for materials and surface science, to achieve the effects of high product sensitivity, simple preparation process and low preparation cost
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Embodiment 1
[0024] A schematic diagram of the preparation process of a ciprofloxacin ratiometric fluorescent probe based on a dual-emission carbon quantum dot / silicon quantum dot nanocomposite and the principle of detection of the ratiometric fluorescence signal of ciprofloxacin involved in this example can be found in figure 1 Shown, its specific process steps are:
[0025] Preparation of silicon quantum dots: 0.5 g of disodium edetate was added to 10 ml of twice-distilled water saturated with argon, and then 2 ml of 3-aminopropyltriethoxysilane was added, magnetically stirred for 10 minutes, and mixed The liquid was transferred to the reaction kettle and reacted at 160°C for 40 minutes. The product was cooled to room temperature and filtered with a microfiltration membrane with a pore size of 0.2 microns. The filtrate was dialyzed with a dialysis bag (molecular weight cut-off 1000 Daltons) to remove residual unreacted Experimental raw materials, take the solution in the dialysis bag, re...
Embodiment 2
[0032] The specific process steps for the preparation of silicon quantum dots and carbon quantum dots in this example are the same as in Example 1. Dissolve the prepared silicon quantum dots in 10 ml of double distilled water, add 20 mg of nitrogen-hydroxysuccinimide and 20 mg of 1-Ethyl-3-(3-dimethylamino)propyl-carbodiimide sodium hydrochloride, stirred magnetically at room temperature for 30 minutes, mixed well, added 10 ml of aqueous solution in which carbon quantum dots were dissolved, and kept in dark place Stirring and reacting for 6 hours, after the reaction, adopt centrifugation, ethanol washing, and vacuum drying to obtain carbon quantum dots / silicon quantum dot nanocomposites, wherein the mass concentration ratio of carbon quantum dots and silicon quantum dots is 1:3; The nanocomposites were dispersed in 10 ml of double distilled water, 10 mg of bis(3-pyridylmethyl)amine, 5 mg of nitrogen-hydroxysuccinimide and 5 mg of 1-ethyl-3-(3-dimethyl Ammonia) propyl-carbodiim...
Embodiment 3
[0034] The specific process steps for the preparation of silicon quantum dots and carbon quantum dots in this example are the same as in Example 1. Dissolve the prepared silicon quantum dots in 10 ml of double distilled water, add 20 mg of nitrogen-hydroxysuccinimide and 20 mg of 1-Ethyl-3-(3-dimethylamino)propyl-carbodiimide sodium hydrochloride, stirred magnetically at room temperature for 30 minutes, mixed well, added 10 ml of aqueous solution in which carbon quantum dots were dissolved, and kept in dark place Stirring and reacting for 6 hours, after the reaction, adopt centrifugation, ethanol washing, and vacuum drying to obtain carbon quantum dots / silicon quantum dot nanocomposites, wherein the mass concentration ratio of carbon quantum dots and silicon quantum dots is 1:5; To 10 ml of double-distilled water in which the nanocomposites were dispersed, add 10 mg of bis(3-pyridylmethyl)amine, 5 mg of nitrogen-hydroxysuccinimide and 5 mg of 1-ethyl-3-(3-dimethyl Ammonia) pro...
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