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A gene diagnostic kit for diagnosing and predicting her-2 overexpression breast cancer bone metastasis

A gene diagnosis, breast cancer technology, applied in the field of biochemistry, can solve the problems of increasing pressure, increasing patient burden, and high inspection costs

Active Publication Date: 2019-01-08
青岛瑞思德医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all have different deficiencies, such as high inspection costs, and interventional diagnosis increases the burden on patients
This increases the pressure on routine detection of bone metastases in breast cancer patients

Method used

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  • A gene diagnostic kit for diagnosing and predicting her-2 overexpression breast cancer bone metastasis
  • A gene diagnostic kit for diagnosing and predicting her-2 overexpression breast cancer bone metastasis
  • A gene diagnostic kit for diagnosing and predicting her-2 overexpression breast cancer bone metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1: Bone Metastasis of Luminal A Breast Cancer

[0078] 1. Experimental samples and experimental methods

[0079] 1. Experimental samples

[0080] The test set and validation set of the non-metastasis group of breast cancer in Luminal A type and the bone metastasis group of breast cancer in Luminal A type.

[0081] 2. RNA extraction and qRT-PCR

[0082] Total RNA was extracted from serum samples using Trizol reagent (Invitrogen, Shanghai, China). The concentration and purity of total RNA were measured at 260nm with a NanoDropND-2000 spectrophotometer (Thermo Scientific). Electrophoresis detection showed that the purified RNA was of good quality and followed up. Total RNA was converted into cDNA using a reverse transcription kit (Takara, Dalian, China). Fluorescent quantitative PCR (Takara, Dalian, China) was carried out using the SYBR Green method, and ABI Prism7000 fluorescent quantitative PCR system (Agilent Technologies) was used for data collection. The ...

Embodiment 2

[0105] Example 2: Bone metastasis of Luminal B breast cancer

[0106] 1. Experimental samples and experimental methods

[0107] 1. Experimental samples

[0108]The test set and validation set of the breast cancer non-metastasis group in Luminal B type and the bone metastasis group of Luminal B breast cancer group.

[0109] 2. RNA extraction and qRT-PCR

[0110] Total RNA was extracted from serum samples using Trizol reagent (Invitrogen, Shanghai, China). The concentration and purity of total RNA were measured at 260nm with a NanoDropND-2000 spectrophotometer (Thermo Scientific). Electrophoresis detection showed that the purified RNA was of good quality and followed up. Total RNA was converted into cDNA using a reverse transcription kit (Takara, Dalian, China). Fluorescent quantitative PCR (Takara, Dalian, China) was carried out using the SYBR Green method, and ABI Prism7000 fluorescent quantitative PCR system (Agilent Technologies) was used for data collection. The primer...

Embodiment 3

[0133] Example 3: Her-2 overexpression breast cancer bone metastasis

[0134] 1. Experimental samples and experimental methods

[0135] 1. Experimental samples

[0136] The test set and validation set of the Her-2 overexpression breast cancer non-metastasis group and the Her-2 overexpression breast cancer bone metastasis group.

[0137] 2. RNA extraction and qRT-PCR

[0138]Total RNA was extracted from serum samples using Trizol reagent (Invitrogen, Shanghai, China). The concentration and purity of total RNA were measured at 260nm with a NanoDropND-2000 spectrophotometer (Thermo Scientific). Electrophoresis detection showed that the purified RNA was of good quality and followed up. Total RNA was converted into cDNA using a reverse transcription kit (Takara, Dalian, China). Fluorescent quantitative PCR (Takara, Dalian, China) was carried out using the SYBR Green method, and ABI Prism7000 fluorescent quantitative PCR system (Agilent Technologies) was used for data collection...

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Abstract

The invention discloses an lncRNA composition as well as application in preparing a kit for diagnosing and predicting Her-2 over-expression breast cancer bone metastasis. According to the invention, serum lncRNAXLOC_004122, SUM01P3 and NBAT-1 can be jointly used for diagnosing and predicting the Luminal A-type breast cancer bone metastasis, the serum lncRNAXLOC_004122, Linc00467 and lncRNAAl049452can be jointly used for diagnosing and predicting the Luminal B-type breast cancer bone metastasis, the serum lncRNAAK043773 and EXOC7 can be jointly used for diagnosing and predicting Her-2 over-expression breast cancer bone metastasis, the lncRNA Lnc01089 and HOTAIR can be jointly used for diagnosing and predicting triple-negative breast cancer bone metastasis, the accuracy is high (90 percentor more), the detection cost is low, and the lncRNA composition is non-invasive, convenient and rapid.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to a diagnostic composition and a diagnostic kit, in particular to an lncRNA diagnostic composition and its application in the preparation of a diagnostic kit for diagnosing and predicting bone metastasis of breast cancer of different molecular subtypes. Background technique [0002] Breast cancer is one of the malignant tumors that threaten women's life and health. About 400,000 to 450,000 people die from breast cancer every year (reference: Clinical characteristics and prognosis analysis of patients with bone metastasis of breast cancer with different molecular subtypes, Journal of Xi'an Jiaotong University· Medical Edition, Volume 38, Issue 5, September 2017). Breast cancer is very prone to distant metastasis, and bone is the most common distant metastasis site of breast cancer, and more than 50% of patients' first metastatic site is bone tissue (reference: Genes associated with breast c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 李宜健薛守海王凤祥刘敏涛胡雨祝
Owner 青岛瑞思德医学检验实验室有限公司
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