Chimeric antigen receptor, gene thereof and recombinant expression vector of gene as well as CARMSLN-NKT cell and preparation method and application of CARMSLN-NKT cell

A chimeric antigen receptor and NKT cell technology, applied in the field of tumor biological products, can solve the problems of insufficient distribution of MSLN monoclonal antibody and transient targeting effect of MSLN monoclonal antibody, achieve good industrial application prospects and enhance specific killing Active, power-enhancing effects
CN107793483AInactive Publication Date: 2018-03-13伍志强
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
伍志强
Publication Date
2018-03-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a chimeric antigen receptor, a gene thereof and a recombinant expression vector of the gene as well as a CARMSLN-NKT cell and a preparation method and application of the CARMSLN-NKT cell. The chimeric antigen receptor is MSLNScFv-CD8-CD137-CD3 zeta including tandem a CD8a signal peptide, an MSLNScFv, shortened CD8 hinge and transmembrane regions, a CD137 intracellular signal domain and a CD3zeta intracellular signal domain; and the CARMSLN-NKT cell provided by the invention has very good specific killing activity against a mesothelioma cell when co-culture is performedwith the MSLN-positive mesothelioma cell.
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Description

technical field

[0001] The invention belongs to the field of tumor biological products, and in particular relates to a chimeric antigen receptor MSLNScFv-CD8-CD137-CD3ζ in adoptive immunotherapy, its gene and recombinant expression vector, and engineered MSLN-targeted NKT cells (CARMSLN -NKT cell) and its preparation method and application. Background technique

[0002] Natural killer cells (NKT) are a special type of T lymphocyte subsets with dual properties of T cells and NK cells. NKT cells can express TCR of T cells and NKR-P1 of NK cells. Under the mediation of TCR and NKR, NKT cells can produce a large amount of IL-4 and INFγ, which can kill tumor cells. NKT cells bind to the Fc region of specific antibodies through CD16 on their surface to exert antibody-dependent cell-mediated cytotoxicity (ADCC, antibody-dependent cell-mediated cytotoxicity). However, in the process of antibody-dependent cell-mediated killing, since the antibody can specifically bind to the corres...

Claims

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