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Xanthomonas oryzae resistance aspergillus sclerotiorum strain As-75 and fermentation culture liquid and application thereof

A technology for fermenting culture broth and Aspergillus nucleatum is applied in the field of biotechnological microorganisms, which can solve the problems of chemical pesticide pollution, influence on rice quality and yield, and easy generation of drug resistance, and achieves the effect of good application prospects.

Active Publication Date: 2018-03-13
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Rice is one of the most important grains in the world, and rice bacterial blight will have a serious impact on rice quality and yield. At present, chemical control is the main method, but chemical pesticides will pollute the environment and easily produce drug resistance. question

Method used

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  • Xanthomonas oryzae resistance aspergillus sclerotiorum strain As-75 and fermentation culture liquid and application thereof
  • Xanthomonas oryzae resistance aspergillus sclerotiorum strain As-75 and fermentation culture liquid and application thereof
  • Xanthomonas oryzae resistance aspergillus sclerotiorum strain As-75 and fermentation culture liquid and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Isolation, Screening and Identification of Aspergillus sclerotiorum As-75 Strain

[0027] 1 medium

[0028] (1) PDA medium: 200 g of potatoes, 20 g of glucose, 20 g of agar powder, 1 L of water, pH 6.5. For the isolation, purification and identification of Aspergillus strains.

[0029] (2) Improved PD culture medium: 200g of potatoes, 20g of glucose, 5g of beef extract, 1L of water, pH 6.5. For the fermentation of Aspergillus strains.

[0030] (3) Rice bacterial blight solid medium (Xoo solid medium): potato 300g, tryptone 5g, sucrose 15g, Ca(NO 3 )·H 2 O 0.5g, Na 2 HPO 4 12H 2 O 2g, agar powder 20g, water 1L, pH 6.5. Used for solid culture of Xanthomonas oryzae.

[0031] (4) rice bacterial blight culture solution (Xoo culture solution): 300g of potatoes, 5g of tryptone, 15g of sucrose, Ca(NO 3 )·H 2 O 0.5g, Na 2 HPO 4 12H 2 O 2g, water 1L, pH 6.5. Used for liquid culture of Xanthomonas oryzae.

[0032] 2 Experimental methods

[0033] 2.1 Prel...

Embodiment 2

[0047] Antimicrobial Spectrum and Stability Measurement of Example 2 Aspergillus sclerotiorum (A.sclerotiorum) As-75 strain

[0048] 1 strain:

[0049] (1) Aspergillus sclerotiorum (A. sclerotiorum) As-75 strain.

[0050] (2) Three kinds of bacteria: Staphylococcus aureus, Escherichia coli, Bacillus subtilis, used for bacterial antibacterial spectrum test.

[0051] (3) 4 kinds of plant pathogenic fungi: wheat scab (Gibberella fujikuroi), tomato early blight (Alternaria solani), poplar wilt (Fusarium solani), cucumber wilt (Fusarium oxysporum f.sp.momordicae), used as Fungal antibacterial spectrum test.

[0052] 2 media

[0053] (1) PDA medium: 200g of potatoes, 20g of glucose, 20g of agar powder, 1L of water, pH 6.0-6.5. It is used for the activation culture of Aspergillus As-75 strain and the culture of three kinds of antibacterial spectrum fungi.

[0054] (2) LB liquid medium: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1L, pH 7.2-7.4. For the cultivation...

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Abstract

The invention belongs to the field of biotechnology microorganism, and relates to a xanthomonas oryzae resistance aspergillus sclerotiorum strain As-75 and application thereof to prevention and control of plant diseases. The strain is preserved in CCTCC (China Center for Type Culture Collection); the preservation address is Wuhan University, Wuhan, China; the preservation code is CCTCC NO:M 2017350; the preservation date is June 19, 2017. The aspergillus sclerotiorum strain As-75 provided by the invention can be used for resisting the xanthomonas oryzae and preparing biological pesticides forpreventing and controlling the xanthomonas oryzae. Meanwhile, a certain inhibition effect is also achieved on bacteria such as staphylococcus aureus and bacillus subtilis; a high inhibition effect isalso achieved on plant pathogenic fungi such as gibberella zeae and poplar oxysporum.

Description

technical field [0001] The invention belongs to the field of biotechnology microorganisms, and relates to an Aspergillus sclerotiorum As-75 strain antagonizing rice bacterial blight and its application in plant disease prevention and treatment. Background technique [0002] Together with rice blast and sheath blight, rice bacterial blight is called the "three major diseases" of rice. Rice bacterial blight, commonly known as white leaf scar, ground fire, etc., is a worldwide bacterial disease caused by Xanthomonas oryzae pv. oryzae (Xoo). After rice suffers from bacterial blight, the yield is generally reduced by 10% to 30%, and the serious disease can reach 50%, or even no harvest; the quality deteriorates, the rice quality is crunchy, and the taste is poor; it seriously threatens global agriculture and food production. Therefore, it is of great significance for agricultural production to seek effective biological control methods to prevent and control the occurrence of bac...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/02A01P1/00A01P3/00C12R1/66
CPCA01N63/10C12N1/14C12N1/145C12R2001/66
Inventor 蒋冬花江北张琪王志颖娄丽倩王林云
Owner ZHEJIANG NORMAL UNIVERSITY
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