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HPLC determination method for measuring content of antipyrine in compound sodium salicylate, antipyrine and caffeine injection

A technology of antipyrine and liuanca, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation, poor accuracy and precision, time-consuming, etc., and achieve the effect of simple operation and accurate results

Inactive Publication Date: 2018-03-23
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sodium salicylate content adopts the acid-base titration method, and the antipyrine content determination method is the reference substance spectrophotometry method, which is complex, time-consuming, and has poor accuracy and precision.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Preparation of reference substance solution: Accurately weigh 70 mg of antipyrine reference substance into a 100 mL measuring bottle, add water to dissolve and set the volume to the mark to prepare antipyrine reference substance solution.

[0016] (2) Preparation of the solution to be tested: take 2ml of the compound Liu'an coffee injection sample, place it in a 500ml measuring bottle, add water to dilute to the mark, then precisely measure 10ml of the solution and place it in a 100ml measuring bottle, add water to dilute to the mark, and obtain The sample solution to be tested.

[0017] (3) formulate standard curve: accurately measure antipyrine reference substance solution 5,7.5,10,12.5,15ml, place respectively in 100ml measuring bottle, add water and dilute to scale, shake up, measure by following chromatographic conditions, Obtain the regression equation of antipyrine: Y=-18925+2314304X (r=0.9999), Y is the peak area, X is the concentration.

[0018] Chromatogr...

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PUM

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Abstract

The invention discloses an HPLC determination method for measuring the content of antipyrine in a compound sodium salicylate, antipyrine and caffeine injection, comprising the following steps: (1) preparation of a reference solution: preparing the reference solution from an antipyrine reference substance and water; (2) preparation of a sample solution to be tested: fetching a compound sodium salicylate, antipyrine and caffeine injection sample and diluting by adding water so as to prepare the sample solution to be tested; (3) establishment of a standard curve: mixing antipyrine reference solutions with different concentrations and conducting quantitative determination by a high performance liquid chromatograph so as to obtain the antipyrine standard curve; and (4) determination of the sample solution to be tested: conducting quantitative determination on the sample solution to be tested by a high performance liquid chromatograph to obtain peak area of sodium salicylate, and obtaining the content of antipyrine in the sample to be tested according to the standard curve of the antipyrine reference substance. The method of the invention is easy to operate, and the result is accurate. The method is suitable for determination of antipyrine in a compound sodium salicylate, antipyrine and caffeine injection.

Description

technical field [0001] The invention relates to a method for determining the content of antipyrine in compound Liu'an coffee injection, in particular to a method for determining the content of antipyrine in compound Liu'an coffee injection by HPLC. Background technique [0002] Compound Liu'anka Injection is mainly used to treat rheumatism, rheumatoid arthritis and other types of arthritis and fever. In the national drug standard, there are only the content determination methods of sodium salicylate and antipyrine of the drug. The sodium salicylate content adopts the acid-base titration method, and the antipyrine content determination method is the reference substance spectrophotometry, and the method is complicated to operate, time-consuming, and has poor accuracy and precision. Contents of the invention [0003] The object of the present invention aims to provide a kind of method of HPLC assaying content of antipyrine in Compound Liu'anka injection. [0004] The object...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 陈学松罗达龙刘慧妍
Owner WUZHOU INST FOR FOOD & DRUG CONTROL