Differentiation inducing agent and method for murine preadipocytes 3T3-L1

A preadipocyte and differentiation-inducing agent technology, which is applied in the direction of cell culture active agent, biochemical equipment and methods, animal cells, etc., can solve the problems of difficulty in inducing differentiation of mouse preadipocyte 3T3-L1 and short differentiation cycle, etc. Achieve the effect of reasonable induction method and steps, short differentiation cycle and reasonable concentration ratio

Active Publication Date: 2018-03-30
上海银海圣生物科技有限公司
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Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of difficult induction and differentiation of mouse preadipocyte 3T3-L1 in the prior art, and to

Method used

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  • Differentiation inducing agent and method for murine preadipocytes 3T3-L1

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Embodiment 1

[0028] The induction and differentiation agents of mouse preadipocyte 3T3-L1 consisted of: 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone, and FBS. The ratio of the above components is: 0.5mmol / L:5μg / mL:1μmol / L:1μmol / L:100mL / L.

Embodiment 2

[0030] The induction and differentiation agents of mouse preadipocyte 3T3-L1 consisted of: 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone, and FBS. The ratio of the above components is: 0.45mmol / L:5.5μg / mL:0.9μmol / L:1.1μmol / L:100mL / L.

Embodiment 3

[0032] The induction and differentiation agents of mouse preadipocyte 3T3-L1 consisted of: 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone, and FBS. The ratio of the above components is: 0.55mmol / L:4.5μg / mL:1.1μmol / L:0.9μmol / L:100mL / L.

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Abstract

The invention provides a differentiation inducing agent and method for murine preadipocytes 3T3-L1. The differentiation inducing agent is prepared from 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone and FBS. The differentiation inducing method comprises the steps that 3T3-L1 cells are cultured in a high-glucose DMEM culture solution containing the differentiation inducing agentafter routine resuscitation culture, then cultured in a high-glucose DMEM culture solution containing insulin and 10% FBS and then cultured in a high-glucose DMEM culture solution containing 10% FBSby means of a half-solution change method. By means of the method, the differentiation cycle is short and is only 6-7 days, the conversion rate of the preadipocytes is high, the cell differentiation is uniform, and the development of studies on fat metabolism diseases is greatly promoted.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to an agent for inducing differentiation of mouse preadipocyte 3T3-L1 and a method for inducing differentiation. Background technique [0002] Mouse preadipocyte 3T3-L1 is a cell line isolated and cloned from mouse embryos with single differentiation potential, which can be induced to differentiate into mature adipocytes under certain conditions. The mature adipocytes differentiated from 3T3-L1 can better mimic the fat cells in the body in terms of morphology and function, so 3T3-L1 is an important tool cell for studying metabolic syndromes such as obesity, diabetes, and non-alcoholic fatty liver. [0003] The cell differentiation process of 3T3-L1 is a comprehensive regulation process of gene level and hormone level. However, 3T3-L1 preadipocytes are not only difficult to culture, but also to induce differentiation and maturation has become the bottleneck of many scientific researches....

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0653C12N2500/40C12N2501/33C12N2501/385C12N2501/39
Inventor 李欣伦江泉肖泽玺祝加和李超刘媛媛张双李秋圆
Owner 上海银海圣生物科技有限公司
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