Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting neomycin sulfate content in triamcinolone acetonide acetat

A neomycin sulfate and assay method technology, applied in the direction of microorganism-based methods, microorganism assay/inspection, biochemical equipment and methods, etc., can solve the problems of poor repeatability of assay results, long assay time, complicated operation, etc. Achieve the effects of easy promotion and application, simple operation, and low experimental cost

Inactive Publication Date: 2018-03-30
广西壮族自治区食品药品检验所
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neomycin sulfate is a multi-component aminoglycoside antibiotic, and its main components are neomycin B and neomycin C. Enterobacteriaceae bacteria such as the genus Primus, Proteus also have good effect, the content assay method of existing neomycin sulfate is complicated to operate, and the assay time is long, and the cost is high, and the repeatability of assay result is poor, therefore, develops a kind of song It is imminent to quickly and accurately determine the content of neomycin sulfate in Mixin cream

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting neomycin sulfate content in triamcinolone acetonide acetat
  • Method for detecting neomycin sulfate content in triamcinolone acetonide acetat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of assay method of neomycin sulfate content in Qumixin emulsifiable cream, comprises the steps:

[0025] (1) Preparation of bacterial suspension

[0026] Take the nutrient agar slant culture of Staphylococcus aureus, inoculate it on the nutrient agar slant, cultivate it at 35°C-37°C for 20-22 hours, wash the bacterial lawn with 0.9% sterilized sodium chloride solution, and set aside;

[0027] (2) Preparation of standard solution

[0028] Accurately weigh 38.35 mg of neomycin sulfate standard product with a content of 652 u / mg into a 25 ml volumetric flask, and dilute to the volume with purified water to obtain a standard solution. Accurately measure the standard solution, and use pH 7.8 phosphoric acid containing 3% sodium chloride Dilute the salt buffer solution into a solution containing 5u and 10u of neomycin sulfate per 1ml;

[0029] (3) Preparation of the test solution

[0030] Accurately weigh 1.67g Qumixin emulsifiable paste in the separatory funnel, ad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting detecting neomycin sulfate content in triamcinolone acetonide acetat. The method comprises the following steps: preparing bacterium suspension; preparinga standard solution; preparing a test solution; preparing a negative sample solution; culturing; detecting. The method is high in specificity and repeatability; compared with a liquid chromatography,the method has the advantages of being simple to operate, low in test cost, and convenient to popularize and apply; dichloromethane is treated as a solvent, so that the solubleness is high, and creamsubstrate can be quickly dissolved in dichloromethane; the influence of the cream substrate is greatly avoided; the detecting neomycin sulfate content detecting result is high in reliability and highin accuracy degree.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for determining the content of neomycin sulfate in Trimethin cream. Background technique [0002] Qumixin cream is a kind of skin medicine, which has significant curative effect on eczema, contact dermatitis, neurodermatitis, seborrheic dermatitis, tinea corporis, jock itch and tinea manus and pedis, etc. The nitric acid contained in Qumixin cream Miconazole is a spectrum antifungal drug, which also has antibacterial effect on some Gram-positive bacteria. Triamcinolone acetonide acetate is a glucocorticoid drug, which has anti-inflammatory, anti-allergic and antipruritic effects for external use. Effective against Gram-positive and negative bacteria. Neomycin sulfate is a multi-component aminoglycoside antibiotic, and its main components are neomycin B and neomycin C. Enterobacteriaceae bacteria such as the genus Primus, Proteus also have good effect, the content assay met...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/445
CPCC12Q1/18G01N2333/31
Inventor 甘永琦农浚樊兰艳朱斌
Owner 广西壮族自治区食品药品检验所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com