Method for detecting neomycin sulfate content in triamcinolone acetonide acetat

A neomycin sulfate and assay method technology, applied in the direction of microorganism-based methods, microorganism assay/inspection, biochemical equipment and methods, etc., can solve the problems of poor repeatability of assay results, long assay time, complicated operation, etc. Achieve the effects of easy promotion and application, simple operation, and low experimental cost

Inactive Publication Date: 2018-03-30
广西壮族自治区食品药品检验所
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Problems solved by technology

Neomycin sulfate is a multi-component aminoglycoside antibiotic, and its main components are neomycin B and neomycin C. Enterobacteriaceae bacteria such as the genus Primus, Proteus also have good effect, the content assay method of existing neomycin sulfate is complicated to operate, and the assay time is long, and the cost is high, and the repeatability of assay result is poor, therefore, develops a kind of song It is imminent to quickly and accurately determine the content of neomycin sulfate in Mixin cream

Method used

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  • Method for detecting neomycin sulfate content in triamcinolone acetonide acetat
  • Method for detecting neomycin sulfate content in triamcinolone acetonide acetat

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Embodiment 1

[0024] A kind of assay method of neomycin sulfate content in Qumixin emulsifiable cream, comprises the steps:

[0025] (1) Preparation of bacterial suspension

[0026] Take the nutrient agar slant culture of Staphylococcus aureus, inoculate it on the nutrient agar slant, cultivate it at 35°C-37°C for 20-22 hours, wash the bacterial lawn with 0.9% sterilized sodium chloride solution, and set aside;

[0027] (2) Preparation of standard solution

[0028] Accurately weigh 38.35 mg of neomycin sulfate standard product with a content of 652 u / mg into a 25 ml volumetric flask, and dilute to the volume with purified water to obtain a standard solution. Accurately measure the standard solution, and use pH 7.8 phosphoric acid containing 3% sodium chloride Dilute the salt buffer solution into a solution containing 5u and 10u of neomycin sulfate per 1ml;

[0029] (3) Preparation of the test solution

[0030] Accurately weigh 1.67g Qumixin emulsifiable paste in the separatory funnel, ad...

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Abstract

The invention provides a method for detecting detecting neomycin sulfate content in triamcinolone acetonide acetat. The method comprises the following steps: preparing bacterium suspension; preparinga standard solution; preparing a test solution; preparing a negative sample solution; culturing; detecting. The method is high in specificity and repeatability; compared with a liquid chromatography,the method has the advantages of being simple to operate, low in test cost, and convenient to popularize and apply; dichloromethane is treated as a solvent, so that the solubleness is high, and creamsubstrate can be quickly dissolved in dichloromethane; the influence of the cream substrate is greatly avoided; the detecting neomycin sulfate content detecting result is high in reliability and highin accuracy degree.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for determining the content of neomycin sulfate in Trimethin cream. Background technique [0002] Qumixin cream is a kind of skin medicine, which has significant curative effect on eczema, contact dermatitis, neurodermatitis, seborrheic dermatitis, tinea corporis, jock itch and tinea manus and pedis, etc. The nitric acid contained in Qumixin cream Miconazole is a spectrum antifungal drug, which also has antibacterial effect on some Gram-positive bacteria. Triamcinolone acetonide acetate is a glucocorticoid drug, which has anti-inflammatory, anti-allergic and antipruritic effects for external use. Effective against Gram-positive and negative bacteria. Neomycin sulfate is a multi-component aminoglycoside antibiotic, and its main components are neomycin B and neomycin C. Enterobacteriaceae bacteria such as the genus Primus, Proteus also have good effect, the content assay met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/445
CPCC12Q1/18G01N2333/31
Inventor 甘永琦农浚樊兰艳朱斌
Owner 广西壮族自治区食品药品检验所
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