Stable reagent for determining glutamic oxalacetic transaminase

Abstract

The invention provides a stable reagent for determining glutamic oxalacetic transaminase. The reagent is a dual reagent and comprises TRIS (tris (hydroxymethyl) aminomethane), NADH (reduced form of nicotinamide-adenine dinucleotid), LDH (lactate dehydrogenase), MDH (malate dehydrogenase), EDTA (ethylene diamine tetraacetic acid), TritonX-100, BSA (bovine serum albumin) and Proclin. According to the reagent, the heat stability, onboard stability and transportation stability of an in-vitro diagnosis reagent of an NADH indicating system are effectively improved; and the shelf life of a reagent kit can be prolonged effectively.

Description

technical field [0001] This application relates to the fields of biochemistry and clinical testing. More specifically, the present application relates to a reagent for the determination of aspartate aminotransferase. Background technique [0002] There are two isoenzymes of aspartate aminotransferase (AST) in the liver, which exist in the mitochondria (mAST) and cytoplasm (sAST) of liver cells respectively. When the liver cells are mildly lesioned, only sAST is released into the blood; and when the lesion is severe, mAST is also released into the blood one after another. Therefore, serum AST activity increases with the degree of liver cell damage. [0003] In HBV-infected hepatitis and liver disease, AST increases slightly with ALT, or the amplitude is relatively large and the time is short, it may be mainly sAST, and the clinical significance is the same as ALT; AST increases more than ALT, although the amplitude is not too large The duration is very long, probably mainl...

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Problems solved by technology

This process also cannot avoid changes in temperature and humidity, and the sample is exposed to air after opening the cover, facing oxidation factors

Therefore, in this case, the accelerated stability test cannot evaluate the stability of other aspects (ISO standard 23640-2011, In vitro diagnostic medical devices-Evaluation of stability of in vitro diagnostic reagents)

[0012] At present, the double-reagent enzyme-coupled continuous detection method is mostly used in the market to detect serum AST. Due to the presence of enzyme and NADH in the reagent, the reagent is unstable during transportation and when the bottle cap is opened and operated on the machine. Serum AST poses difficulties

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