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Stable reagent for determining glutamic oxalacetic transaminase

A technology of aspartate aminotransferase and reagents, which is applied in the fields of biochemistry and clinical testing, and can solve problems such as difficulty in detecting serum AST, accelerated stability experiments, and unstable reagents

Active Publication Date: 2018-03-30
BEIJING BEIJIAN XINCHUANGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process also cannot avoid changes in temperature and humidity, and the sample is exposed to air after opening the cover, facing oxidation factors
Therefore, in this case, the accelerated stability test cannot evaluate the stability of other aspects (ISO standard 23640-2011, In vitro diagnostic medical devices-Evaluation of stability of in vitro diagnostic reagents)
[0012] At present, the double-reagent enzyme-coupled continuous detection method is mostly used in the market to detect serum AST. Due to the presence of enzyme and NADH in the reagent, the reagent is unstable during transportation and when the bottle cap is opened and operated on the machine. Serum AST poses difficulties

Method used

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  • Stable reagent for determining glutamic oxalacetic transaminase
  • Stable reagent for determining glutamic oxalacetic transaminase
  • Stable reagent for determining glutamic oxalacetic transaminase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: the preparation of comparison kit

[0065] First Reagent R1:

[0066]

[0067] Second reagent R2:

[0068] TRIS, pH6.5 100mmol / L

[0069] L-Aspartic Acid 720mmol / L

[0070] α-ketoglutarate 36mmol / L

Embodiment 2

[0071] Embodiment 2: the preparation of test kit

[0072] The preparation method of the second reagent R2 is the same as in Example 1; the preparation method of the first reagent R1 is shown in Table 1.

[0073] Table 1. R1 reagent composition of different test kits

[0074]

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PUM

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Abstract

The invention provides a stable reagent for determining glutamic oxalacetic transaminase. The reagent is a dual reagent and comprises TRIS (tris (hydroxymethyl) aminomethane), NADH (reduced form of nicotinamide-adenine dinucleotid), LDH (lactate dehydrogenase), MDH (malate dehydrogenase), EDTA (ethylene diamine tetraacetic acid), TritonX-100, BSA (bovine serum albumin) and Proclin. According to the reagent, the heat stability, onboard stability and transportation stability of an in-vitro diagnosis reagent of an NADH indicating system are effectively improved; and the shelf life of a reagent kit can be prolonged effectively.

Description

technical field [0001] This application relates to the fields of biochemistry and clinical testing. More specifically, the present application relates to a reagent for the determination of aspartate aminotransferase. Background technique [0002] There are two isoenzymes of aspartate aminotransferase (AST) in the liver, which exist in the mitochondria (mAST) and cytoplasm (sAST) of liver cells respectively. When the liver cells are mildly lesioned, only sAST is released into the blood; and when the lesion is severe, mAST is also released into the blood one after another. Therefore, serum AST activity increases with the degree of liver cell damage. [0003] In HBV-infected hepatitis and liver disease, AST increases slightly with ALT, or the amplitude is relatively large and the time is short, it may be mainly sAST, and the clinical significance is the same as ALT; AST increases more than ALT, although the amplitude is not too large The duration is very long, probably mainl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 姜敏
Owner BEIJING BEIJIAN XINCHUANGYUAN BIOLOGICAL TECH
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