A strain of Bacillus coagulans e21 that improves the anti-oxidative stress ability of livestock and poultry animals after challenge and its application
A Bacillus coagulans, anti-oxidative stress technology, applied in applications, animal feed, animal feed, etc., can solve the problems of anti-bile salt and poor high temperature resistance, and achieve good effect and good heat resistance
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Embodiment 1
[0021] The strain comes from the cecal digesta of healthy chickens. After multiple screening and purification tests, the pure strain of the bacteria can be obtained. The bacteria can produce acid and spores. The DNA of the bacteria is extracted after the pure bacteria are cultured in liquid medium, and then 16S Universal primers were used to amplify the full length of 16S rRNA, and the amplified product was sent to a sequencing company for sequencing. The sequencing results were compared with Bacillus coagulans and found to be 99% similar to Bacillus coagulans, confirming that the bacterium was Bacillus coagulans. The whole genome of the strain was sequenced using the third-generation sequencing PacBio technology, and it was found that the strain was inconsistent with the strains on the market, and was identified as Bacillus coagulans E21.
[0022] The physiological and biochemical properties of the Bacillus coagulans E21 screened by the present invention are as follows: Gram-p...
Embodiment 2
[0032] In this experiment, diquat, a bipyridine herbicide, was used to attack white-feathered broiler chickens. The specific operations are as follows:
[0033] The test selects 1-day-old white-feathered broiler chickens, pre-feeds them for 7 days, removes the chicks with large body weight differences, and the rest are grouped. The test is divided into 3 treatment groups, and each treatment group has 4 repetitions (i.e. 4 stalls). Repeat 15 chickens, and 3 groups are challenged and processed in the following way:
[0034] Control group: without adding any probiotics, on the 21st day of the test, the same amount of normal saline was injected;
[0035] Test group 1: without adding any probiotics, on the 21st day of the test, intraperitoneal injection of diquat (15 mg / kg body weight, diquat dilution concentration of 15 mg / mL);
[0036] Test group 2: Add 10 6 CFU / mL of Bacillus coagulans, on the 21st day of the test, inject diquat (15mg / kg body weight, diquat dilution concentrat...
Embodiment 3
[0061] The Bacillus coagulans obtained in the present invention is fermented and cultivated. The fermentation of the bacteria utilizes a 15L fermenter, the fermentation period is 24-30h, and the amount of fermented bacteria reaches 10 billion CFU / mL, wherein the formula of the fermentation medium is: soybean meal 3-5%, Peptone 1-2%, cornmeal 3-5%, glucose 1-1.5%, dipotassium hydrogen phosphate 0.3-0.5%, manganese sulfate 0.02-0.1%, sodium nitrate 0.05-0.1%. 20 billion CFU / g Bacillus coagulans powder can be obtained after post-treatment and other processes after fermentation. The Bacillus coagulans powder can be used in combination with other probiotics, or used alone in feed, or fed to broilers Drinking water is added during the process and used alone.
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