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A kind of pea rhizobia strain vs13-1 and its application

A technology of arrowhead pea and rhizobia, applied in the field of microorganisms, to achieve the effects of good affinity, strong stress resistance, and obvious increase in production

Inactive Publication Date: 2020-06-02
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on high-efficiency rhizobia suitable for Sichuan ecological environment is almost blank

Method used

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  • A kind of pea rhizobia strain vs13-1 and its application
  • A kind of pea rhizobia strain vs13-1 and its application
  • A kind of pea rhizobia strain vs13-1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation, Purification and Preservation of Pea Rhizobium Arrowhead VS13-1

[0016] From the wild arrowhead peas collected in Tiemenkan Village, Fuxing Town, Yilong County, Nanchong City, Sichuan Province, select the large and full red root nodules on the main roots of robust plants, wash the root nodules, and pick off some root bark, and carry out surface disinfection: use 95% Soak in ethanol for 5 minutes to eliminate surface tension, then sterilize the surface with mercury chloride (0.1% m / v) for 5 minutes, and finally rinse with sterile water for 6-8 times. Put the root nodules on a sterilized white porcelain plate under a sterile environment, clip the root nodules with a bamboo stick and smear them on the plate of YMA medium, perform streaking and purification, and culture in a 28°C incubator. YMA medium formula: KH 2 PO 4 0.25g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 ·6H 2 O 0.1g, NaCl 0.1g, ammonium molybdate (1%) 2mL, boric acid (1%) 2mL, Congo red (1%) 2....

Embodiment 2

[0019] The back graft test of embodiment 2 rhizobia

[0020] The backgrafting test of rhizobia uses the sand culture method, and the arrowhead pea variety used in the test is North Sichuan arrowhead pea, which comes from Xiangshui Village, Yanjiang Town, Yanjiang District, Ziyang City, Sichuan. Carry out in the light room (temperature control 22~24 ℃, light intensity about 2800 lux, sunshine time 14h), plant and harvest 46 days. Regularly supplement the sterilized nitrogen-free nutrient solution, inoculate the rhizobia VS13-1 into the sand culture device, the sand culture device uses a 300mL plastic cup, and use vermiculite as the substrate, so as not to inoculate the rhizobia VS13-1 The plants of the same variety as the control CK, each treatment was repeated three times. After harvesting, the nodule number and dry weight of the plants were used to evaluate the inoculation effect of Rhizobia VS13-1.

[0021] (1) Bacterial liquid culture: inoculate Rhizobium spp. VS13-1 in Y...

Embodiment 3

[0031] Example 3 Stress resistance of Pea rhizobia VS13-1

[0032] The stress resistance of Rhizobium spp. VS13-1 was mainly tested for salt tolerance, acid and alkali resistance. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control (CK). Scrape and wash the above-mentioned YMA slant culture of Rhizobium argentina VS13-1 with sterile water to make a bacterial suspension for use. The spot inoculation method was used, and each treatment was repeated 3 times. Plates for acid and alkali resistance and salt resistance tests were all cultured at 28°C for 3 days, and the results were observed and recorded.

[0033] (1) Medium formula for acid and alkali resistance determination: take YMA medium as the basic medium, adjust the pH value with HCl and NaOH, so that the pH value of the medium is 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, 11.0 .

[0034] (2) Medium formula for salt tolerance determination: take the YMA...

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Abstract

The invention discloses a pea rhizobium strain VS13‑1 and application thereof. The strain is isolated and purified from fresh pea rhizobium and belongs to Rhizobium anhuiense strain. The strain was deposited in the Chinese Type Culture Collection Center of Wuhan University on October 23, 2017, and the preservation number is: CCTCC No: M 2017607. The pea rhizobia VS13-1 described in the present invention is applied to the production of peas in Sichuan. It is a kind of symbiotic nitrogen-fixing ability, which has an obvious effect on increasing the production of peas in quail. It also has the ability to secrete IAA, dissolve phosphorus, dissolve The excellent rhizobia strain of Arrowhead pea with strong potassium ability and stress resistance can increase the fresh grass yield of Arrowhead pea by more than 76.5% and the fresh root yield by 68.7% without applying nitrogen fertilizers or other fertilizers and only inoculating rhizobia VS13‑1 .

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain VS13-1 of Pisa rhizobium and application thereof. Background technique [0002] Arrowhead pea (Vicia sativa L.), also known as Vicia sativa L., belongs to a cultivar of the genus Vetaceae of the family Leguminosae. It has a large main root, shallow penetration into the soil, many root nodules, and arrow-shaped leaves, so it is named Arrowhead Pea. Pea, which originated in southern Europe and western Asia, was introduced into my country for planting and cultivation in the 1940s. The planting range in my country reached 29 provinces (regions) including Qinghai, Jiangsu, Hunan, and Taiwan (Hong Ruxing et al., 1985). Arrowhead pea, as an excellent feed and green manure dual-purpose crop, has high nutritional value, tender stems and branches, more leaves, and good palatability. It is a relatively common green manure germplasm resource used in production (Lu Binglin et al., 2015; Zhao H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01C1/06C12R1/41
CPCA01C1/06C12N1/205C12R2001/41
Inventor 陈远学徐开未龙玲彭丹全紫曼胡月秋陈松鹤
Owner SICHUAN AGRI UNIV
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