Accurate packing and mixing device for DNA primers or probes and using method of accurate packing and mixing device

Abstract

The invention discloses a precise dispensing and mixing device for DNA primers or probes and a method for using the same. The platen and the fixed platen are composed; the actuator is composed of the X-axis slide table, the Y-axis slide table, the Z-axis slide table and the gun axis slide table; the working area components include 8 sub-assembly target boards, 1 gun head board, 1 A stock solution plate, a liquid guide target plate and a pipette tip separator are provided, and the worktable adopts a layout with the stock solution plate as the center. The present invention provides an accurate, reliable and efficient engineering solution, so as to realize automatic and precise dispensing and mixing of DNA primers or probes. The present invention can save labor and tedious manual liquid suction and pumping process. The operator only needs to use a mobile U disk to import the required operation into the software, saves the previous preparation work, and realizes the fully automatic operation.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a precise packaging and mixing device for DNA primers or probes and a method for using the same. Background technique [0002] In molecular biology research, primers with different base sequences and lengths need to be synthesized according to specific purposes. After the DNA primer is synthesized, it is usually placed in a standard well plate, such as a 48-well plate or a 96-well plate, and each well can contain a different primer. According to the different purposes of analysis and research, sometimes it is necessary to distribute the same synthesized primer or probe (called one) into several different centrifuge tubes or screw tubes, and sometimes it is necessary to distribute different primers or probes The needles are mixed into the same tube, usually these dispensing or mixing work is done manually by the operator with the aid of a pipette. Completin...

AI Technical Summary

Problems solved by technology

Because the primers or probes that need to be packaged or mixed are measured in microliters, the operator’s skills are very high, and it is often difficult to control the measurement deviation or fluctuation in a small range

At the same time, manual operation cannot completely avoid human errors, and it is difficult to find errors when they occur

[0003] The laboratory pipetting workstation or nucleic acid extractor in the prior art can only achieve the purpose of uniformly transferring a certain liquid from one container to another, but cannot realize the complex and complex process of subpackaging and mixing primers or probes. Sophisti

Images