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Detection method for residue of clenobuterol hydrochloride

A detection method, the technology of clenbuterol, which is applied in the detection field of clenbuterol residues, can solve the problems of expensive instruments, long time, false negatives, etc., and achieve the effects of simple operation, promotion of distribution, and good dispersibility

Inactive Publication Date: 2018-04-20
闫博文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention: Although the enzyme-linked immunoassay in the current method for detecting clenbuterol residues is sensitive and fast, it is easy to produce false positives and false negatives. Although the method has high-efficiency separation and detection capabilities, the instrument is expensive, and gas chromatography has the advantages of high sensitivity, but it often needs to be derivatized during the determination, and the pretreatment process takes a long time. A detection method for clenbuterol residues is provided.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Extraction of Clenbuterol samples to be tested: accurately weigh the pork and add 5% HClO with a mass ratio of 1:3 4 Solution, homogenize to obtain a homogenate, take the standard Clenbuterol hydrochloride and mix with methanol at a mass ratio of 1:150 to obtain a stock solution. Take the homogenate and add it to the stock solution at a mass ratio of 10:3, vortex and mix for 3 minutes, then Ultrasound for 20 minutes, centrifuge at 12000r / min for 10 minutes, take out the supernatant and add n-hexane at a mass ratio of 5:1, stir and mix for 3 minutes, centrifuge, and remove the clear liquid to obtain a sample of clenbuterol for testing.

[0030] C18 solid phase extraction column (100mg, 1mL): purchased from Suzhou Saifen Technology Co., Ltd.

[0031] A method for detecting clenbuterol residues includes the following steps:

[0032] (1) Take graphite powder and add 70% sulfuric acid with a mass ratio of 1:40, stir and mix at -1°C for 15 minutes, then add 15% potassium permanganat...

Embodiment 2

[0038] Extraction of Clenbuterol samples to be tested: accurately weigh the pork and add 5% HClO with a mass ratio of 1:3 4 Solution, homogenize to obtain a homogenate. Take the standard Clenbuterol hydrochloride and mix with methanol at a mass ratio of 1:150 to obtain a stock solution. Take the homogenate and add it to the stock solution at a mass ratio of 10:3, vortex and mix for 4 minutes, and then Ultrasound for 25min, centrifuge at 12000r / min for 10min, take out the supernatant and add n-hexane at a mass ratio of 5:1, stir and mix for 4min, centrifuge, remove the clear liquid, and obtain the sample of clenbuterol for testing.

[0039] C18 solid phase extraction column (100mg, 1mL): purchased from Suzhou Saifen Technology Co., Ltd.

[0040] A method for detecting clenbuterol residues includes the following steps:

[0041] (1) Take graphite powder and add 70% sulfuric acid at a mass ratio of 1:40, stir and mix at -1°C for 17 minutes, then add 70% sulfuric acid with 16% potassium p...

Embodiment 3

[0047] Extraction of Clenbuterol samples to be tested: accurately weigh the pork and add 5% HClO with a mass ratio of 1:3 4 Solution, homogenize to obtain a homogenate. Take the standard Clenbuterol hydrochloride and mix with methanol at a mass ratio of 1:150 to obtain a stock solution. Take the homogenate and add it to the stock solution at a mass ratio of 10:3, vortex and mix for 4 minutes, and then Ultrasound for 25min, centrifuge at 12000r / min for 10min, take out the supernatant and add n-hexane at a mass ratio of 5:1, stir and mix for 4min, centrifuge, remove the clear liquid, and obtain the sample of clenbuterol for testing.

[0048] C18 solid phase extraction column (100mg, 1mL): purchased from Suzhou Saifen Technology Co., Ltd.

[0049] A method for detecting clenbuterol residues includes the following steps:

[0050] (1) Take graphite powder and add 70% sulfuric acid with a mass ratio of 1:40, stir and mix for 17 minutes at 0°C, then add 17% potassium permanganate with a mas...

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Abstract

The invention belongs to analysis methods, in particular to a detection method for clenbuterol residues. The present invention combines the excellent properties of graphene and ferroferric oxide nanoparticles, has good dispersibility, huge specific surface area, super magnetism and excellent extraction ability, utilizes hydrothermal synthesis, uses water instead of organic solvent, and can prepare single Dispersed particles are uniform nanoparticles, and the prepared composite surface has many functional groups that can be further chemically modified. It is used as a magnetic adsorbent, and the magnetic adsorbent in the sample is directly separated by an external magnetic field without additional centrifugation, filtration, etc. steps, effectively shorten the analysis time, overcome the time-consuming problem of traditional solid phase extraction, and it can also be recovered and reused.

Description

Technical field [0001] The invention belongs to an analysis method, and specifically relates to a method for detecting clenbuterol residues. Background technique [0002] The country is based on the people, food is the priority for the people, and food is the first. In recent years, the frequent occurrence of food safety incidents has severely threatened the health of consumers. Among them, since 2001, the Guangdong clenbuterol incident caused hundreds of people to be poisoned, making clenbuterol into the eyes of people. Subsequently, clenbuterol poisoning incidents occurred in Taiwan, Shanghai and many other places, especially the "Shuanghui" clenbuterol incident in 2011 has aroused widespread concern. [0003] Clenbuterol is a kind of adrenal nerve stimulant, specifically a type of β2-receptor agonist, which can selectively excite β2-adrenergic receptors, such as ractopamine, crotren, and salbutamol. Such molecules can relax bronchial smooth muscle, and mast cells have been rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/062
Inventor 闫博文郑薇林凡
Owner 闫博文
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