Establishment of diagnostic model for liver cancer and normal human and diagnostic model for liver cancer and chronic liver disease

Abstract

The invention discloses establishment of a diagnostic model for liver cancer and normal human and a diagnostic model for liver cancer and chronic liver disease. An SELDI-TOF-MS technology is used, thediagnostic model for liver cancer and normal human and the diagnostic model for liver cancer and chronic liver disease are established; sera of 48 cases of patients with liver cancer, 17 cases of patients with chronic liver disease and 33 cases of healthy people are collected; the SELDI-TOF technology is used for detecting the samples; searching of effective tumor markers for researches of canceration mechanism and early diagnosis and prognosis judgment of diseases is of great significance. The effects of blocking or up-regulation of expression of candidate genes on tumor biological behaviorsare observed, and key proteins affecting generation and development of human liver cancer are positioned; through bioinformatics analysis, the role of the key proteins in a system signal network is analyzed; through MRM absolute quantification verification, the value of liver cancer diagnosis of liver cancer key protein polypeptides is evaluated and the clinical relevance is analyzed, and the feasibility of the key proteins in clinical applications, such as prevention, early diagnosis and prognosis of liver cancer, is provided.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to establishing a diagnostic model for liver cancer and normal people and a diagnostic model for liver cancer and chronic liver disease. Background technique [0002] In the 20th century, primary liver cancer has risen to become the second cancer killer in my country, second only to lung cancer in urban areas and gastric cancer in rural areas [1] . At present, about 35,000 people in Guangxi die of malignant tumors every year, among which, about 12,000 people die of liver cancer. It can be seen that liver cancer is the first cancer killer in Guangxi. In Guangxi, the hazards of liver cancer are very uneven. The counties and cities with high mortality rate of liver cancer are mainly concentrated in the southwestern part of Guangxi. The three counties of Fusui, Long'an and Wuming around Nanning have the highest incidence. The incidence density of liver cancer in thi...

AI Technical Summary

Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry

Although researchers have tried to use a variety of methods, including chromatographic separation as much as possible, removal of high-abundance proteins, etc., the precision and accuracy of the results are still far from meeting the requirements of analysis, until mass spectrometry multiple reaction monitoring (multiple reaction monitoring) The emergence of monitoring, MRM) technology

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